Kara P Pelin, Ayhan Ali, Caner Biray, Gultekin Murat, Ugur Omer, Bozkurt M Fani, Usubutun Alp, Uner Aysegul
Department of Nuclear Medicine, Hacettepe University Faculty of Medicine, Sihhiye, Ankara, Turkey.
Int J Gynecol Cancer. 2009 Oct;19(7):1239-43. doi: 10.1111/IGC.0b013e3181b3e616.
The aim of this study was to identify the immune response in sentinel lymph nodes (SLNs) of patients with endometrial and patients with cervical cancers by analyzing the number of S-100-, CD1a-, CD83-positive (+) dendritic cells that are the major antigen-presenting cells.
A total of 56 patients with early-stage cancer (n = 32, with cervical; n = 24, with endometrial cancer) underwent SLN biopsy. Sentinel lymph nodes and non-SLNs were stained with antibodies against S-100, CD1a, and CD83 as markers for dendritic cells to find out whether SLNs were immunomodulated compared with non-SLNs.
The mean values of S-100(+) and CD1a(+) dendritic cells in both the tumor-free and the metastatic SLNs were significantly higher than those of both the tumor-free and the metastatic non-SLNs. When metastatic SLNs were compared with nonmetastatic SLNs, CD83(+) dendritic cells were found significantly more abundant in nonmetastatic SLNs.
Significantly higher numbers of S-100(+) and CD1a(+) dendritic cells in the SLNs compared with those in the non-SLNs may indicate that SLNs are the first sites of immunostimulation. Immunosupression may be the underlying factor for the metastatic involvement of SLNs, which might be secondary to the significantly decreased number of mature dendritic cells in metastatic SLNs compared with tumor-free SLNs.
本研究旨在通过分析作为主要抗原呈递细胞的S-100、CD1a、CD83阳性（+）树突状细胞的数量，确定子宫内膜癌和宫颈癌患者前哨淋巴结（SLN）中的免疫反应。
共有56例早期癌症患者（n = 32例宫颈癌患者；n = 24例子宫内膜癌患者）接受了前哨淋巴结活检。用抗S-100、CD1a和CD83抗体对前哨淋巴结和非前哨淋巴结进行染色，作为树突状细胞的标志物，以确定前哨淋巴结与非前哨淋巴结相比是否受到免疫调节。
无瘤前哨淋巴结和转移前哨淋巴结中S-100（+）和CD1a（+）树突状细胞的平均值均显著高于无瘤和转移的非前哨淋巴结。当将转移前哨淋巴结与非转移前哨淋巴结进行比较时，发现非转移前哨淋巴结中CD83（+）树突状细胞明显更多。
与非前哨淋巴结相比，前哨淋巴结中S-100（+）和CD1a（+）树突状细胞数量显著增加，可能表明前哨淋巴结是免疫刺激的首个部位。免疫抑制可能是前哨淋巴结发生转移的潜在因素，这可能继发于转移前哨淋巴结中成熟树突状细胞数量与无瘤前哨淋巴结相比显著减少。
