两种使用单克隆抗体检测N1,N8 - 二乙酰亚精胺和N1,N12 - 二乙酰精胺的酶联免疫吸附测定（ELISA）系统。

Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies.

作者信息

Hamaoki Masaru, Hiramatsu Kyoko, Suzuki Setsuo, Nagata Atsuo, Kawakita Masao

机构信息

Immunology Laboratory, Diagnostics Department, Yamasa Corporation, Araoi-cho, Choshi, Chiba 288-0056.

出版信息

J Biochem. 2002 Nov;132(5):783-8. doi: 10.1093/oxfordjournals.jbchem.a003287.

DOI:10.1093/oxfordjournals.jbchem.a003287

PMID:12417029

Abstract

We obtained monoclonal antibodies against N(1),N(12)-diacetylspermine (DiAcSpm) and N(1),N(8)-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these di-acetylpolyamines. Cross-reactions with N(1)-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetylpolyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation.

摘要

我们获得了针对N(1),N(12)-二乙酰精胺（DiAcSpm）和N(1),N(8)-二乙酰亚精胺（DiAcSpd）的单克隆抗体，并开发了两种竞争性酶联免疫吸附测定（ELISA）系统，该系统利用这些抗体和一种常见的酶标记抗原来测量这些二乙酰多胺。在DiAcSpm测定中与N(1)-乙酰亚精胺以及在DiAcSpd测定中与N8-乙酰亚精胺的交叉反应分别低至0.26%和0.6%，并且在临床用于测量尿中二乙酰多胺时被判定为无显著影响。这些测定用于评估尿中二乙酰多胺排泄的昼夜变化，结果表明，以肌酐浓度进行标准化后，二乙酰多胺的排泄在一天内是稳定的，昼夜变化极小。