Hiramatsu K, Sugimoto M, Kamei S, Hoshino M, Kinoshita K, Iwasaki K, Kawakita M
Tokyo Metropolitan Institute of Medical Science.
J Biochem. 1995 Jan;117(1):107-12. doi: 10.1093/oxfordjournals.jbchem.a124694.
An analytical system developed for fractionating free and monoacetylated polyamines [Hiramatsu, K. et al. (1994) J. Biochem. 115, 584-589] was proved useful also in detecting diacetylpolyamines, namely N1,N8-diacetylspermidine (diAcSpd) and N1,N12-diacetylspermine (diAcSpm). Detection limits were 0.9 and 0.6 pmol (S/N = 5) for diAcSpd and diAcSpm, respectively. Analytical recovery and within-run variation were also satisfactory. Human urine samples were found to contain diAcSpd and diAcSpm. These polyamines were identified on the basis of the following observations: (i) their retention times were coincident with those of authentic samples; (ii) they were deacetylated to N8-acetylspermidine and monoacetyl- and free spermine, respectively, by acetylpolyamine amidohydrolase; and (iii) they were practically inert to direct oxidation by bacterial polyamine oxidase as were authentic samples. The amounts of eleven polyamine species including diAcSpd and diAcSpm in urine samples from 52 healthy persons were determined. Mean values for the major polyamine components were consistent with those reported by others. Although the amounts of diAcSpd and diAcSpm were very small, comprising only 1.4 and 0.46% of total polyamines, respectively, these two compounds were found to be always present in healthy human urine as regular constituents. Moreover, variation in their content among individuals was small, suggesting that excretion of these components in urine is strictly regulated.
一种用于分离游离和单乙酰化多胺的分析系统[Hiramatsu, K.等人(1994年)《生物化学杂志》115卷，584 - 589页]经证实也可用于检测二乙酰化多胺，即N1,N8 - 二乙酰亚精胺(diAcSpd)和N1,N12 - 二乙酰精胺(diAcSpm)。diAcSpd和diAcSpm的检测限分别为0.9和0.6皮摩尔(S/N = 5)。分析回收率和批内变异也令人满意。发现人类尿液样本中含有diAcSpd和diAcSpm。这些多胺基于以下观察结果得以鉴定：(i)它们的保留时间与真实样品的保留时间一致；(ii)它们分别被乙酰多胺酰胺水解酶脱乙酰化为N8 - 乙酰亚精胺以及单乙酰化和游离的精胺；(iii)它们与真实样品一样，对细菌多胺氧化酶的直接氧化实际上呈惰性。测定了52名健康人尿液样本中包括diAcSpd和diAcSpm在内的11种多胺种类的含量。主要多胺成分的平均值与其他人报道的一致。尽管diAcSpd和diAcSpm的含量非常少，分别仅占总多胺的1.4%和0.46%，但发现这两种化合物在健康人尿液中始终作为常规成分存在。此外，个体之间它们含量的差异很小，这表明尿液中这些成分的排泄受到严格调节。
