Ohnishi Ken, Ota Ichiro, Yane Katsunari, Takahashi Akihisa, Yuki Kazue, Emoto Mie, Hosoi Hiroshi, Ohnishi Takeo
Department of Biology, Nara Medical University, Kashihara, Nara, 634-8521, Japan.
Mol Cancer. 2002 Oct 4;1:4. doi: 10.1186/1476-4598-1-4.
Anaplastic thyroid carcinoma, which is one of the most aggressive, malignant tumors in humans, results in an extremely poor prognosis despite chemotherapy and radiotherapy. The present study was designed to evaluate therapeutic effects of radiation by glycerol on p53-mutant anaplastic thyroid carcinoma cells (8305c cells). To examine the effectiveness of glycerol in radiation induced lethality for anaplastic thyroid carcinoma 8305c cells, we performed colony formation assay and apoptosis analysis.
Apoptosis was analyzed with Hoechst 33342 staining and DNA ladder formation assay. 8305c cells became radiosensitive when glycerol was added to culture medium before X-ray irradiation. Apoptosis was induced by X-rays in the presence of glycerol. However, there was little apoptosis induced by X-ray irradiation or glycerol alone. The binding activity of whole cell extracts to bax promoter region was induced by X-rays in the presence of glycerol but not by X-rays alone.
These findings suggest that glycerol is effective against radiotherapy of p53-mutant thyroid carcinomas.
间变性甲状腺癌是人类最具侵袭性的恶性肿瘤之一，尽管进行了化疗和放疗，其预后仍然极差。本研究旨在评估甘油对p53突变的间变性甲状腺癌细胞（8305c细胞）的放射治疗效果。为了检测甘油在辐射诱导间变性甲状腺癌8305c细胞致死性方面的有效性，我们进行了集落形成试验和凋亡分析。
用Hoechst 33342染色和DNA梯状条带形成试验分析凋亡情况。在X射线照射前向培养基中添加甘油时，8305c细胞对辐射变得敏感。在甘油存在的情况下，X射线诱导了凋亡。然而，单独的X射线照射或甘油几乎不诱导凋亡。在甘油存在的情况下，X射线诱导全细胞提取物与bax启动子区域的结合活性，但单独的X射线不诱导。
这些发现表明甘油对p53突变型甲状腺癌的放射治疗有效。
