Meulemans G, van den Berg T P, Decaesstecker M, Boschmans M
Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium.
Avian Pathol. 2002 Oct;31(5):515-9. doi: 10.1080/0307945021000005897.
Twenty-seven Newcastle disease virus isolates obtained during the years 1998 and 1999 from racing pigeons were shown to be antigenically indistinguishable from the pigeon paramyxovirus type 1 (PPMV-1) viruses isolated in the years 1983 and 1984. Partial sequencing of 240 base pairs of the F gene demonstrated at least 94.7% identity at the nucleotide level between isolates from 1983 and 1984, and more recent viruses isolated in 1998 and 1999. Most of the nucleotide changes observed were silent mutations as only six amino acid changes were observed. Three amino acid substitutions were observed in the F2/F1 cleavage site. The sequence of the F2/F1 cleavage site of all isolates was typical for pathogenic paramyxovirus 1 viruses. Amino acids at the F2/F1 cleavage site changed from (112)GRQKRF(117) to (112)RRQKRF(117), (112)RRKKRF(117) or (112)RRRKRF(117). The motif (112)RRQKRF(117) was present in the majority of the isolates but the intracerebral pathogenicity indexes of PPMV-1 isolates having this motif was highly variable but largely lower (mean, 0.69) than that reported for PPMV-1 viruses isolated in the years 1983 and 1984 (mean, 1.44).
1998年和1999年期间从赛鸽中分离得到的27株新城疫病毒，在抗原性上与1983年和1984年分离得到的1型鸽副粘病毒（PPMV-1）病毒没有区别。对F基因240个碱基对进行的部分测序表明，1983年和1984年分离的毒株与1998年和1999年分离的较新病毒在核苷酸水平上至少有94.7%的同一性。观察到的大多数核苷酸变化是沉默突变，因为仅观察到6个氨基酸变化。在F2/F1裂解位点观察到3个氨基酸替换。所有分离株F2/F1裂解位点的序列对于致病性副粘病毒1型病毒来说是典型的。F2/F1裂解位点的氨基酸从（112）GRQKRF（117）变为（112）RRQKRF（117）、（112）RRKKRF（117）或（112）RRRKRF（117）。大多数分离株中存在基序（112）RRQKRF（117），但具有该基序的PPMV-1分离株的脑内致病性指数变化很大，但大多低于1983年和1984年分离的PPMV-1病毒所报告的水平（平均值为0.69，而1983年和1984年分离的PPMV-1病毒平均值为1.44）。
