Rola-Plezczynski M, Hensen S A, Vincent M M, Bellanti J A
J Pediatr. 1975 May;86(5):690-6. doi: 10.1016/s0022-3476(75)80352-0.
A significant depression in cell-mediated immunity as measured by lymphoproliferative responses to phytohemagglutinin and responsiveness to mixed lymphocyte culture was observed when adult lymphocytes or cord blood lymphocytes were incubated with increasing concentrations of bilirubin. The inhibitory effect of bilirubin could only be demonstrated with suboptimal concentrations of PHA (0.01 and 0.005%) and was more marked in premature infants than in term neonates or adults. This effect was partially reversible after short preincubation with bilirubin, but was more protracted with preincubations of 24 hours or more. Inhibition of MLC responsiveness of 80.1 plus or minus 5.1% was also demonstrated at a bilirubin concentration of 20 mg/dl. Specific cytotoxicity to rubella virus-infected cells, measured by a 51Cr-release microassay, was not found to be depressed. Bilirubin thus appears to have an inhibitory effect on immune responsiveness which is greater on the afferent limb than on the effrent limb of immunity.
当成年淋巴细胞或脐血淋巴细胞与浓度不断增加的胆红素一起孵育时，通过对植物血凝素的淋巴细胞增殖反应和对混合淋巴细胞培养的反应性所测量的细胞介导免疫出现显著抑制。胆红素的抑制作用仅在次优浓度的PHA（0.01%和0.005%）下才能表现出来，并且在早产儿中比足月儿或成年人中更为明显。在与胆红素进行短时间预孵育后，这种作用部分可逆，但在预孵育24小时或更长时间时更为持久。在胆红素浓度为20mg/dl时，也证实对混合淋巴细胞培养反应性的抑制为80.1±5.1%。通过51Cr释放微量测定法测量，未发现对风疹病毒感染细胞的特异性细胞毒性降低。因此，胆红素似乎对免疫反应性具有抑制作用，对免疫传入支的抑制作用大于传出支。
