实验性胆汁淤积中特异性细胞免疫受损。
Impaired nonspecific cellular immunity in experimental cholestasis.
作者信息
Roughneen P T, Drath D B, Kulkarni A D, Rowlands B J
机构信息
Department of Surgery, University of Texas Medical School, Houston 77030.
出版信息
Ann Surg. 1987 Nov;206(5):578-82. doi: 10.1097/00000658-198711000-00004.
The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C5a and FMLP, phagocytosis of 14C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p less than 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of 14C labeled Staphylococcus aureus was depressed in BDL (p less than 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p less than 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice.
研究了大鼠胆管结扎后多形核白细胞（PMN）和肺泡巨噬细胞（PAM）表现出趋化性、吞噬作用和超氧化物释放的能力，以确定胆汁淤积对非特异性细胞免疫机制的影响。在胆管结扎（BDL）、假手术或正常对照21天后，评估对C5a和FMLP的趋化反应、14C标记金黄色葡萄球菌的吞噬作用以及酵母聚糖诱导的超氧化物释放。BDL后血清总胆红素水平升高（p<0.01）。趋化能力在各组之间相似。BDL组中PMN对14C标记金黄色葡萄球菌的吞噬摄取降低（p<0.05）。BDL大鼠表现出PAM吞噬指数受损和PMN超氧化物释放增加（p<0.03）。各研究组中PAM超氧化物释放相似。胆汁淤积时吞噬功能的改变是非特异性细胞免疫中的重要缺陷，可能导致与梗阻性黄疸相关的感染性并发症的高发生率。
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