Chen Bin, Pei Guo-Xian, Wang Ke, Fan Yi-Xiang, Wang Hong, Jin Dan, Wei Kuan-Hai
Department of Orthopedics and Traumatology, First Military Medical University, Guangzhou, 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):966-9.
To observe the effect of tissue-engineered bone grafts in repairing large tibial defect in goats, and assess the value of radionuclide bone imaging in monitoring the therapeutic effect of this approach.
Tibial defects measuring 2 cm was artificially made in the left tibia of 27 normal goats that were subsequently divided into 3 groups (9 each) to undergo treatment with tissue-engineered bone grafts, artificial bone grafts or without any grafts (as control group) respectively. The tissue-engineered bone grafts contained bone marrow stroma cells (BMSCs) of the goats and coral hydroxyapatite (CHAP), while the artificial bone grafts were from CHAP only. After the operations, radionuclide bone imaging was used to monitor the therapeutic effects at 2, 4 and 8 postoperative weeks.
The 99mTc-MDP uptake of the region of interest (ROI) and the target to non-target ratios (T/NT) of the control group did not indicate any process of revascularization or bone regeneration. An increasing tendency of the revascularization and bone regeneration, in contrast, was observed in goats receiving the artificial bone grafts, a tendency that was far more obvious in the goats with tissue-engineered bone grafts.
Tissue-engineered bone graft is eligible in repairing large defect in the caprine tibia, and radionuclide bone imaging may accurately monitor the revascularization and bone regeneration after the bone graft implantation.
观察组织工程骨移植修复山羊胫骨大段缺损的效果，并评估放射性核素骨显像在监测该方法治疗效果中的价值。
在27只正常山羊的左胫骨上人工制造2 cm的胫骨缺损，随后将其分为3组（每组9只），分别接受组织工程骨移植、人工骨移植或不进行任何移植（作为对照组）治疗。组织工程骨移植包含山羊骨髓基质细胞（BMSCs）和珊瑚羟基磷灰石（CHAP），而人工骨移植仅来自CHAP。术后，在术后2、4和8周使用放射性核素骨显像监测治疗效果。
对照组感兴趣区（ROI）的99mTc-MDP摄取量和靶非靶比值（T/NT）未显示任何血管再生或骨再生过程。相比之下，接受人工骨移植的山羊观察到血管再生和骨再生有增加趋势，在接受组织工程骨移植的山羊中这种趋势更为明显。
组织工程骨移植适用于修复山羊胫骨大段缺损，放射性核素骨显像可准确监测骨移植植入后的血管再生和骨再生情况。
