Lartigue Marie Frédérique, Leflon-Guibout Véronique, Poirel Laurent, Nordmann Patrice, Nicolas-Chanoine Marie-Hélène
Service de Microbiologie-Hygiène, Hôpital Ambroise Paré Assistance Publique-Hôpitaux de Paris, Boulogne, France.
Antimicrob Agents Chemother. 2002 Dec;46(12):4035-7. doi: 10.1128/AAC.46.12.4035-4037.2002.
Using an isogenic system, we have determined the impact that the four promoters known to control bla(TEM) gene expression have on beta-lactamase activity. For both TEM-1 and TEM-30, this activity gradually increased in relation to the presence of promoters P3, Pa/Pb, and P4 upstream of the corresponding gene. Promoter P5, only found upstream of the bla(TEM-1B) gene, was related to the highest expression of this gene.
利用同基因系统,我们已确定已知控制bla(TEM)基因表达的四个启动子对β-内酰胺酶活性的影响。对于TEM-1和TEM-30,相对于相应基因上游启动子P3、Pa/Pb和P4的存在,这种活性逐渐增加。仅在bla(TEM-1B)基因上游发现的启动子P5与该基因的最高表达相关。