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在244位具有不同取代的耐抑制剂TEM（IRT）β-内酰胺酶。

Inhibitor-resistant TEM (IRT) beta-lactamases with different substitutions at position 244.

作者信息

Bret L, Chaibi E B, Chanal-Claris C, Sirot D, Labia R, Sirot J

机构信息

Laboratoire de Bactériologie, Faculté de Médecine, Clermont-Ferrand, France.

出版信息

Antimicrob Agents Chemother. 1997 Nov;41(11):2547-9. doi: 10.1128/AAC.41.11.2547.

DOI:10.1128/AAC.41.11.2547
PMID:9371365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC164160/
Abstract

A novel inhibitor-resistant TEM (IRT) beta-lactamase was detected in an Escherichia coli isolate resistant to amoxicillin-clavulanate and susceptible to cephalothin. The substrate and inhibitor profiles of this beta-lactamase were similar to those of IRT-1 and IRT-2. The novel IRT's bla gene was sequenced, and the deduced amino acid sequence showed the amino acid replacement Arg for His-244 of the TEM-1 sequence. Substitutions for Arg-244 have been reported in three TEM-1 mutants: IRT-1 (which corresponds to TEM-31) (Cys), IRT-2/TEM-30 (Ser), and TEM-41 (Thr). We designated this novel beta-lactamase, which corresponds to TEM-51, IRT-15.

摘要

在一株对阿莫西林-克拉维酸耐药但对头孢噻吩敏感的大肠埃希菌分离株中检测到一种新型的抑制剂耐药性TEM（IRT）β-内酰胺酶。该β-内酰胺酶的底物和抑制剂谱与IRT-1和IRT-2相似。对该新型IRT的bla基因进行了测序，推导的氨基酸序列显示在TEM-1序列的His-244位点发生了氨基酸替换，由精氨酸取代。在三个TEM-1突变体中已报道了Arg-244的替换：IRT-1（对应于TEM-31）（半胱氨酸）、IRT-2/TEM-30（丝氨酸）和TEM-41（苏氨酸）。我们将这种对应于TEM-51的新型β-内酰胺酶命名为IRT-15。

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本文引用的文献

1
Characterization of an inhibitor-resistant enzyme IRT-2 derived from TEM-2 beta-lactamase produced by Proteus mirabilis strains.奇异变形杆菌菌株产生的源自TEM-2β-内酰胺酶的抑制剂抗性酶IRT-2的特性分析
J Antimicrob Chemother. 1996 Aug;38(2):183-91. doi: 10.1093/jac/38.2.183.
2
First characterization of inhibitor-resistant TEM (IRT) beta-lactamases in Klebsiella pneumoniae strains.肺炎克雷伯菌菌株中耐抑制剂TEM（IRT）β-内酰胺酶的首次鉴定。
Antimicrob Agents Chemother. 1995 Nov;39(11):2580-2. doi: 10.1128/AAC.39.11.2580.
3
Incidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli.大肠杆菌对阿莫西林与克拉维酸联合用药的耐药发生率及机制
Antimicrob Agents Chemother. 1995 Nov;39(11):2478-83. doi: 10.1128/AAC.39.11.2478.
4
Characterization of a new TEM-type beta-lactamase resistant to clavulanate, sulbactam, and tazobactam in a clinical isolate of Escherichia coli.一株临床分离的大肠杆菌中对克拉维酸、舒巴坦和他唑巴坦耐药的新型TEM型β-内酰胺酶的特性分析
Antimicrob Agents Chemother. 1993 Oct;37(10):2059-63. doi: 10.1128/AAC.37.10.2059.
5
Effect of hyperproduction of TEM-1 beta-lactamase on in vitro susceptibility of Escherichia coli to beta-lactam antibiotics.TEM-1β-内酰胺酶过度产生对大肠杆菌体外对β-内酰胺类抗生素敏感性的影响。
Antimicrob Agents Chemother. 1994 Mar;38(3):494-8. doi: 10.1128/AAC.38.3.494.
6
Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors.产生TEM-1衍生物或对β-内酰胺酶抑制剂耐药的OXA-1β-内酰胺酶的大肠杆菌临床分离株的出现。
Antimicrob Agents Chemother. 1994 May;38(5):1085-9. doi: 10.1128/AAC.38.5.1085.
7
Nucleotide sequences of the genes coding for the TEM-like beta-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2).编码TEM样β-内酰胺酶IRT-1和IRT-2（以前称为TRI-1和TRI-2）的基因的核苷酸序列。
FEMS Microbiol Lett. 1994 Jul 1;120(1-2):75-80. doi: 10.1111/j.1574-6968.1994.tb07010.x.
8
Characterization and amino acid sequence of IRT-4, a novel TEM-type enzyme with a decreased susceptibility to beta-lactamase inhibitors.IRT-4的特性及氨基酸序列，一种对β-内酰胺酶抑制剂敏感性降低的新型TEM型酶
FEMS Microbiol Lett. 1994 Jul 1;120(1-2):111-7. doi: 10.1111/j.1574-6968.1994.tb07016.x.
9
Clinical isolates of Escherichia coli producing multiple TEM mutants resistant to beta-lactamase inhibitors.产生对β-内酰胺酶抑制剂耐药的多个TEM突变体的大肠杆菌临床分离株。
J Antimicrob Chemother. 1994 Jun;33(6):1117-26. doi: 10.1093/jac/33.6.1117.
10
Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coli.来自大肠杆菌临床分离株的107株耐抑制剂TEMβ-内酰胺酶中9种不同类型突变体的分子特征分析。
Antimicrob Agents Chemother. 1995 Feb;39(2):427-30. doi: 10.1128/AAC.39.2.427.