Chuh Antonio An Tung, Chan Henry Hin Lee, Chiu Susan Shui Seng, Ng Hoi Yee, Peiris Joseph Sriyal Malik
Department of Medicine, University of Hong Kong and Queen Mary Hospital, Pokfulam, Hong Kong, SAR.
Pediatr Dermatol. 2002 Nov-Dec;19(6):492-7. doi: 10.1046/j.1525-1470.2002.00217.x.
Gianotti-Crosti syndrome (GCS) is known to be associated with hepatitis B and Epstein-Barr virus (EBV) infections. Apart from a single case report based on serology alone, there are no published data on an association between GCS and human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) primary infections or reactivations. Our aim was to investigate the association between GCS and HHV-6 and HHV-7 infections. Ten patients diagnosed with GCS at a primary care practice over an 18-month period were recruited. Controls were age- and sex-matched patients with unrelated symptoms requiring venepuncture for other indications. Blood specimens were collected from patients and controls at presentation, and from patients 4 weeks later. Virologic evidence of HHV-6 and HHV-7 infection was sought in peripheral blood leukocytes and plasma using polymerase chain reaction (PCR) for viral DNA, reverse transcriptase polymerase chain reaction (RT-PCR) for HHV-6 U91 mRNA transcripts, and serology. Serology for EBV and hepatitis B virus was done. In contrast to the 10 controls, 2 patients (both infants) with clinically diagnosed GCS had evidence of active HHV-6 infection. This was demonstrated by detection of viral DNA in the absence of antibody in the acute plasma specimens and HHV-6 DNA viral loads of more than 5.3 log10 genome copies/5 microl in the whole blood specimens, a profile previously shown to be diagnostic of recent primary HHV-6 infection. None of the patients had evidence of recent EBV or hepatitis B infection. We conclude that primary HHV-6 infection may be associated with GCS in some infants.
已知 Gianotti-Crosti 综合征（GCS）与乙型肝炎和 Epstein-Barr 病毒（EBV）感染有关。除了一篇仅基于血清学的个案报告外，尚无关于 GCS 与人类疱疹病毒 6（HHV-6）和人类疱疹病毒 7（HHV-7）原发性感染或再激活之间关联的已发表数据。我们的目的是研究 GCS 与 HHV-6 和 HHV-7 感染之间的关联。招募了在 18 个月期间在基层医疗诊所被诊断为 GCS 的 10 名患者。对照组为年龄和性别匹配、因其他指征需要静脉穿刺且有无关症状的患者。在就诊时从患者和对照组采集血液标本，并在 4 周后从患者采集。使用聚合酶链反应（PCR）检测病毒 DNA、针对 HHV-6 U91 mRNA 转录本的逆转录聚合酶链反应（RT-PCR）以及血清学方法，在外周血白细胞和血浆中寻找 HHV-6 和 HHV-7 感染的病毒学证据。进行了 EBV 和乙型肝炎病毒的血清学检测。与 10 名对照组患者不同，2 名临床诊断为 GCS 的患者（均为婴儿）有活动性 HHV-6 感染的证据。这通过在急性血浆标本中检测到无抗体情况下的病毒 DNA 以及全血标本中 HHV-6 DNA 病毒载量超过 5.3 log10 基因组拷贝数/5 微升得以证明，这种情况先前已被证明可诊断为近期原发性 HHV-6 感染。所有患者均无近期 EBV 或乙型肝炎感染的证据。我们得出结论，原发性 HHV-6 感染可能在一些婴儿中与 GCS 有关。
