Jayaprakasha Guddadarangavvanahally K, Jena Bhabani S, Negi Pradeep S, Sakariah Kunnumpurath K
Human Resource Development, Central Food Technological Research Institute, Mysore, India.
Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):828-35. doi: 10.1515/znc-2002-9-1013.
Curcumin removed turmeric oleoresin (CRTO) was extracted with hexane concentrated to get turmeric oil, and that was fractionated using silica gel column chromatography to obtain three fractions. These fractions were analyzed by GC and GC-MS. Turmeric oil contained aromatic turmerone (31.32%), turmerone (15.08%) and curlone (9.7%), whereas fractions III has aromatic turmerone (44.5%), curlone (19.22%) and turmerone (10.88%) as major compounds Also, oxygenated compounds (5,6,8-10) were enriched in fraction III. Turmeric oil and its fractions were tested for antioxidant activity using the beta-carotene-linoleate model system and the phosphomolybdenum method. The fraction III showed maximum antioxidant capacity. These fractions were also used to determine their protective effect against the mutagenicity of sodium azide by means of the Ames test. All the fractions and turmeric oil exhibited a markedly antimutagenicity but fraction III was the most effective. The antioxidant effects of turmeric oil and its fractions may provide an explanation for their antimutagenic action.
用己烷萃取除去姜黄素的姜黄油树脂（CRTO），浓缩后得到姜黄油，再用硅胶柱色谱法对其进行分离得到三个馏分。采用气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）对这些馏分进行分析。姜黄油中含有芳姜黄酮（31.32%）、姜黄酮（15.08%）和姜烯酮（9.7%），而馏分III中主要成分是芳姜黄酮（44.5%）、姜烯酮（19.22%）和姜黄酮（10.88%）。此外，馏分III中富含氧化合物（5,6,8 - 10）。使用β - 胡萝卜素 - 亚油酸酯模型体系和磷钼酸法对姜黄油及其馏分的抗氧化活性进行测试。馏分III表现出最大的抗氧化能力。还通过艾姆斯试验利用这些馏分来测定它们对叠氮化钠致突变性的保护作用。所有馏分和姜黄油均表现出显著的抗诱变活性，但馏分III最为有效。姜黄油及其馏分的抗氧化作用可能为它们的抗诱变作用提供一种解释。
