Vladić Anton, Horvat Gordana, Vukadin Stjepan, Sucić Zvonimir, Simaga Sumski
Department of Neurology, General Hospital Sveti Duh, Zagreb, Croatia.
Cytokine. 2002 Oct 21;20(2):86-9. doi: 10.1006/cyto.2002.1984.
The aim of this study was to evaluate soluble proteins of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-6 receptor subunit gp80 (sIL-6R gp80), as markers of multiple sclerosis (MS). Paired cerebrospinal fluid (CSF) and serum samples of 20 MS patients and 15 controls suffering from non-inflammatory neurological diseases have been assayed retrospectively using monoclonal antibodies-based ELISAs. While TNF-alpha could not be detected in CSF, it was measurable in 20% of total sera. Interleukin-6 was measurable in 5% of total CSF and in 10% of total sera only. However, soluble IL-6R gp80 protein subunit was readily measurable, showing sera concentration (pg/mL) about 34 times higher and specific content (pg/mg total protein) around five times lower than those in paired CSF, similarly for both group of patients. No significant difference of sIL-6R gp80 level, which could be disease-, gender- or age-related, and no correlation of CSF sIL-6R gp80 content with that of paired serum or with routine clinical data for CSF, have been observed. We have concluded that soluble proteins of TNF-alpha, IL-6 and sIL-6R gp80 assayed by monoclonal antibodies-based ELISAs could not serve as markers of the MS activity.
本研究旨在评估肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和IL-6受体亚基gp80（sIL-6R gp80）的可溶性蛋白作为多发性硬化症（MS）标志物的情况。我们使用基于单克隆抗体的酶联免疫吸附测定法（ELISA）对20例MS患者和15例患有非炎性神经系统疾病的对照者的配对脑脊液（CSF）和血清样本进行了回顾性检测。虽然在CSF中未检测到TNF-α，但在20%的血清样本中可检测到。白细胞介素-6仅在5%的CSF样本和10%的血清样本中可检测到。然而，可溶性IL-6R gp80蛋白亚基易于检测，在两组患者中，血清浓度（pg/mL）比配对CSF中的浓度高约34倍，而比配对CSF中的浓度低约五倍，同样，血清中总蛋白含量（pg/mg总蛋白）也低于配对CSF中的含量。未观察到sIL-6R gp80水平存在与疾病、性别或年龄相关的显著差异，且未观察到CSF中sIL-6R gp80含量与配对血清中的含量或CSF常规临床数据之间存在相关性。我们得出结论，通过基于单克隆抗体的ELISA检测的TNF-α、IL-6和sIL-6R gp80的可溶性蛋白不能作为MS活动的标志物。
