Wang Liang, Han Yujing, Zhou Shuo, Wang Guihua, Guan Xiyun
Department of Biological and Chemical Sciences, Illinois Institute of Technology , 3101 South Dearborn Street, Chicago, Illinois 60616, United States.
ACS Appl Mater Interfaces. 2014 May 28;6(10):7334-9. doi: 10.1021/am500749p. Epub 2014 May 14.
We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.
我们报告了一种无标记实时纳米孔传感方法，用于通过使用互补单链DNA作为分子探针来检测炭疽致死因子（炭疽毒素的一种成分）。该方法快速且灵敏：在约1分钟内可检测到亚纳摩尔浓度的目标炭疽致死因子DNA。此外，我们的方法具有选择性，能够以单碱基分辨率将目标DNA与其他单链DNA分子区分开来。这种序列特异性检测方法在开发用于检测其他病原体的纳米孔传感器方面应会有有用的应用。
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