• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于无标记实时检测炭疽致死因子的纳米孔生物传感器。

Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.

作者信息

Wang Liang, Han Yujing, Zhou Shuo, Wang Guihua, Guan Xiyun

机构信息

Department of Biological and Chemical Sciences, Illinois Institute of Technology , 3101 South Dearborn Street, Chicago, Illinois 60616, United States.

出版信息

ACS Appl Mater Interfaces. 2014 May 28;6(10):7334-9. doi: 10.1021/am500749p. Epub 2014 May 14.

DOI:10.1021/am500749p
PMID:24806593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4039345/
Abstract

We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.

摘要

我们报告了一种无标记实时纳米孔传感方法，用于通过使用互补单链DNA作为分子探针来检测炭疽致死因子（炭疽毒素的一种成分）。该方法快速且灵敏：在约1分钟内可检测到亚纳摩尔浓度的目标炭疽致死因子DNA。此外，我们的方法具有选择性，能够以单碱基分辨率将目标DNA与其他单链DNA分子区分开来。这种序列特异性检测方法在开发用于检测其他病原体的纳米孔传感器方面应会有有用的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/5365261c3f56/am-2014-00749p_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/691ee853dbe5/am-2014-00749p_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/a606c07e9e88/am-2014-00749p_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/6c8104d8b8d1/am-2014-00749p_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/4c3108afd81e/am-2014-00749p_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/5365261c3f56/am-2014-00749p_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/691ee853dbe5/am-2014-00749p_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/a606c07e9e88/am-2014-00749p_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/6c8104d8b8d1/am-2014-00749p_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/4c3108afd81e/am-2014-00749p_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b7a/4039345/5365261c3f56/am-2014-00749p_0005.jpg

相似文献

1
Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.用于无标记实时检测炭疽致死因子的纳米孔生物传感器。
ACS Appl Mater Interfaces. 2014 May 28;6(10):7334-9. doi: 10.1021/am500749p. Epub 2014 May 14.
2
Detection of anthrax lef with DNA-based photonic crystal sensors.基于 DNA 的光子晶体传感器检测炭疽杆菌。
J Biomed Opt. 2011 Dec;16(12):127006. doi: 10.1117/1.3662460.
3
Nanopore-Assisted, Sequence-Specific Detection, and Single-Molecule Hybridization Analysis of Short, Single-Stranded DNAs.纳米孔辅助的短单链 DNA 的序列特异性检测和单分子杂交分析。
Anal Chem. 2019 Jul 2;91(13):8630-8637. doi: 10.1021/acs.analchem.9b02080. Epub 2019 Jun 13.
4
Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach.金纳米粒子-蛋白质纳米孔法序列特异性检测单链 DNA。
Sci Rep. 2020 Jul 9;10(1):11323. doi: 10.1038/s41598-020-68258-x.
5
Nonfunctionalized PNAs as Beacons for Nucleic Acid Detection in a Nanopore System.非功能化 PNAs 作为纳米孔系统中核酸检测的信标。
ACS Sens. 2019 Jun 28;4(6):1502-1507. doi: 10.1021/acssensors.9b00553. Epub 2019 May 28.
6
Evaluation study protocols and acceptance criteria for hand held assays intended for detection of Bacillus anthracis.用于检测炭疽芽孢杆菌的手持式检测方法的评估研究方案及验收标准。
J AOAC Int. 2005 Jan-Feb;88(1):13A-14A.
7
One-pot synthesis of strongly fluorescent DNA-CuInS quantum dots for label-free and ultrasensitive detection of anthrax lethal factor DNA.一锅法合成强荧光DNA-CuInS量子点用于炭疽致死因子DNA的无标记超灵敏检测。
Anal Chim Acta. 2016 Oct 26;942:86-95. doi: 10.1016/j.aca.2016.09.004. Epub 2016 Sep 8.
8
Label-free dual sensing of DNA molecules using GaN nanowires.使用氮化镓纳米线对DNA分子进行无标记双传感
Anal Chem. 2009 Jan 1;81(1):36-42. doi: 10.1021/ac800986q.
9
A rapid and sensitive detection of HBV DNA using a nanopore sensor.使用纳米孔传感器快速灵敏地检测乙肝病毒DNA。
Chem Commun (Camb). 2014 Nov 18;50(89):13853-6. doi: 10.1039/c4cc06135g.
10
Electrically active magnetic nanoparticles as novel concentrator and electrochemical redox transducer in Bacillus anthracis DNA detection.电活性磁性纳米粒子作为新型浓缩剂和电化学氧化还原转换器在炭疽芽孢杆菌 DNA 检测中的应用。
Biosens Bioelectron. 2010 Dec 15;26(4):1624-30. doi: 10.1016/j.bios.2010.08.035. Epub 2010 Aug 20.

引用本文的文献

1
Nanopore Environmental Analysis.纳米孔环境分析
JACS Au. 2025 Apr 1;5(4):1570-1590. doi: 10.1021/jacsau.5c00114. eCollection 2025 Apr 28.
2
Sensing Microorganisms Using Rapid Detection Methods: Supramolecular Approaches.使用快速检测方法传感微生物：超分子方法
Biosensors (Basel). 2025 Feb 21;15(3):130. doi: 10.3390/bios15030130.
3
Nanopore-based detection of periodontitis biomarker miR31 in saliva samples.基于纳米孔的唾液样本中牙周炎生物标志物miR31的检测

本文引用的文献

1
Investigation of Cu2+ binding to human and rat amyloid fragments Aβ (1-16) with a protein nanopore.利用蛋白质纳米孔研究铜离子与人和大鼠淀粉样片段Aβ(1 - 16)的结合
Langmuir. 2013 Dec 17;29(50):15634-42. doi: 10.1021/la403915t. Epub 2013 Dec 6.
2
A chemically synthesized capture agent enables the selective, sensitive, and robust electrochemical detection of anthrax protective antigen.一种化学合成的捕获剂使炭疽保护性抗原的选择性、灵敏性和稳健的电化学检测成为可能。
ACS Nano. 2013 Oct 22;7(10):9452-60. doi: 10.1021/nn404296k. Epub 2013 Oct 8.
3
Detection of protective antigen, an anthrax specific toxin in human serum by using surface plasmon resonance.
Electrophoresis. 2024 Nov;45(21-22):2034-2044. doi: 10.1002/elps.202400134. Epub 2024 Aug 21.
4
A highly sensitive nanopore platform for measuring RNase A activity.一种用于测量 RNase A 活性的高灵敏度纳米孔平台。
Talanta. 2024 Aug 15;276:126276. doi: 10.1016/j.talanta.2024.126276. Epub 2024 May 22.
5
Probe-assisted detection of Fe ions in a multi-functionalized nanopore.探针辅助检测多功能纳米孔中的 Fe 离子。
Biosens Bioelectron. 2024 May 1;251:116125. doi: 10.1016/j.bios.2024.116125. Epub 2024 Feb 12.
6
Nanopore Single-molecule Analysis of Biomarkers: Providing Possible Clues to Disease Diagnosis.生物标志物的纳米孔单分子分析：为疾病诊断提供可能线索
Trends Analyt Chem. 2023 May;162. doi: 10.1016/j.trac.2023.117060. Epub 2023 Apr 11.
7
Single-molecule Study on the Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore.环非核糖体肽与蛋白质纳米孔相互作用的单分子研究
ACS Appl Bio Mater. 2020 Jan 21;3(1):554-560. doi: 10.1021/acsabm.9b00961. Epub 2019 Dec 16.
8
A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange.一种用于无泵样品交换的杯上脂质双层装置。
Micromachines (Basel). 2020 Dec 18;11(12):1123. doi: 10.3390/mi11121123.
9
Salt-Mediated Nanopore Detection of ADAM-17.盐介导的ADAM-17纳米孔检测
ACS Appl Bio Mater. 2019 Jan 22;2(1):504-509. doi: 10.1021/acsabm.8b00689. Epub 2018 Dec 24.
10
Assessing the Potential Deployment of Biosensors for Point-of-Care Diagnostics in Developing Countries: Technological, Economic and Regulatory Aspects.评估生物传感器在发展中国家即时诊断中的潜在应用：技术、经济和监管方面。
Biosensors (Basel). 2018 Nov 29;8(4):119. doi: 10.3390/bios8040119.
利用表面等离子体共振技术检测人血清中的炭疽保护性抗原，一种炭疽特异性毒素。
Diagn Microbiol Infect Dis. 2013 Sep;77(1):14-9. doi: 10.1016/j.diagmicrobio.2013.05.006. Epub 2013 Jun 15.
4
Nanopore stochastic detection: diversity, sensitivity, and beyond.纳米孔随机检测：多样性、灵敏度及其他。
Acc Chem Res. 2013 Dec 17;46(12):2867-77. doi: 10.1021/ar400031x. Epub 2013 Apr 24.
5
Electrical graphene aptasensor for ultra-sensitive detection of anthrax toxin with amplified signal transduction.基于电石墨烯适体传感器的炭疽毒素超高灵敏检测及其信号放大。
Small. 2013 Oct 11;9(19):3352-60. doi: 10.1002/smll.201203245. Epub 2013 Apr 16.
6
Probing mercury(II)-DNA interactions by nanopore stochastic sensing.通过纳米孔随机传感探测汞（II）-DNA 相互作用。
J Phys Chem B. 2013 May 2;117(17):4763-9. doi: 10.1021/jp309541h. Epub 2013 Apr 23.
7
Designing a polycationic probe for simultaneous enrichment and detection of microRNAs in a nanopore.设计一种用于在纳米孔中同时富集和检测 microRNAs 的聚阳离子探针。
ACS Nano. 2013 May 28;7(5):3962-9. doi: 10.1021/nn305789z. Epub 2013 Apr 10.
8
A novel homogeneous immunoassay for anthrax detection based on the AlphaLISA method: detection of B. anthracis spores and protective antigen (PA) in complex samples.一种基于 AlphaLISA 方法的新型炭疽检测均相免疫分析：复杂样品中炭疽芽孢杆菌孢子和保护性抗原（PA）的检测。
Anal Bioanal Chem. 2013 May;405(12):3965-72. doi: 10.1007/s00216-013-6752-1. Epub 2013 Feb 7.
9
Recent advances in bacteriophage based biosensors for food-borne pathogen detection.基于噬菌体的生物传感器在食源性病原体检测中的最新进展。
Sensors (Basel). 2013 Jan 30;13(2):1763-86. doi: 10.3390/s130201763.
10
Recent advances in molecular medicine techniques for the diagnosis, prevention, and control of infectious diseases.分子医学技术在传染病的诊断、预防和控制方面的最新进展。
Eur J Clin Microbiol Infect Dis. 2013 Jun;32(6):723-8. doi: 10.1007/s10096-013-1813-0. Epub 2013 Jan 22.