Sambol Susan P, Merrigan Michelle M, Tang Janet K, Johnson Stuart, Gerding Dale N
Infectious Disease Section, Department of Medicine, Veterans Affairs Chicago Health Care System, Lakeside Division, Chicago, Illinois, USA.
J Infect Dis. 2002 Dec 15;186(12):1781-9. doi: 10.1086/345676. Epub 2002 Nov 22.
Studies suggest that asymptomatic colonization with Clostridium difficile (CD) decreases the risk of CD-associated disease (CDAD) in humans. A hamster model was used to test the efficacy of colonization with 3 nontoxigenic CD strains for preventing CDAD after exposure to toxigenic CD. Groups of 10 hamsters were given 10(6) nontoxigenic CD spores 2 days after receiving a single dose of clindamycin. Five days later, the hamsters were given 100 spores of 1 of 3 toxigenic CD strains previously shown to cause mortality within 48 h. Each nontoxigenic strain prevented disease in 87%-97% of hamsters that were challenged with toxigenic strains. Failure to prevent CDAD was associated with failure of colonization with nontoxigenic CD. Colonization with nontoxigenic CD strains is highly effective in preventing CDAD in hamsters challenged with toxigenic CD strains, which suggests that use of a probiotic strategy for CDAD prevention in humans receiving antibiotics might be beneficial.
研究表明，艰难梭菌（CD）无症状定植可降低人类患CD相关疾病（CDAD）的风险。使用仓鼠模型来测试3种非产毒CD菌株的定植对暴露于产毒CD后预防CDAD的效果。每组10只仓鼠在接受单剂量克林霉素2天后给予10(6)个非产毒CD孢子。五天后，给仓鼠接种先前显示在48小时内可导致死亡的3种产毒CD菌株之一的100个孢子。每种非产毒菌株在87%-97%受到产毒菌株攻击的仓鼠中预防了疾病。未能预防CDAD与非产毒CD定植失败有关。非产毒CD菌株的定植在预防受到产毒CD菌株攻击的仓鼠发生CDAD方面非常有效，这表明在接受抗生素治疗的人类中使用益生菌策略预防CDAD可能有益。
