Songer J Glenn, Jones Randy, Anderson Michael A, Barbara Angelique J, Post Karen W, Trinh Hien T
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, AZ 85721, USA.
Vet Microbiol. 2007 Oct 6;124(3-4):358-61. doi: 10.1016/j.vetmic.2007.04.019. Epub 2007 Apr 8.
Clostridium difficile is widely known as a cause of disease in humans, and has emerged as an important problem in neonatal swine. No commercial product is available for immunoprophylaxis of C. difficile-associated disease, but success in preventing experimental infections in hamsters by use of nontoxigenic strains to competitively exclude toxigenic strains led us to try this method in neonatal pigs. Spores were administered orally to newborn pigs or were sprayed onto perineum and teats of dams. Significantly more piglets were weaned among litters receiving spores orally, and average weaning weights were significantly higher for both treatment groups than for controls. Toxins A and B were detected in 44.8% of litters and 16.5% of piglets born to sprayed sows and 58.3% of litters and 15.4% of piglets in the control group. However, toxins were detected in only 13.8% of litters and 3.4% of piglets given spores orally. These data support a contention that precolonization by a nontoxigenic strain can ameliorate the pre-weaning growth retardation associated with C. difficile infection in piglets.
艰难梭菌作为人类疾病的病因广为人知，并且已成为新生仔猪中的一个重要问题。目前尚无用于艰难梭菌相关疾病免疫预防的商业产品，但通过使用非产毒菌株竞争性排除产毒菌株来预防仓鼠实验性感染取得成功，这促使我们在新生仔猪中尝试这种方法。将孢子口服给予新生仔猪，或将其喷洒在母猪的会阴和乳头上。口服孢子的窝中成功断奶的仔猪明显更多，两个治疗组的平均断奶体重均显著高于对照组。在喷洒孢子的母猪所产仔猪中，44.8%的窝和16.5%的仔猪检测到毒素A和B，对照组中为58.3%的窝和15.4%的仔猪。然而，口服孢子的仔猪中仅13.8%的窝和3.4%的仔猪检测到毒素。这些数据支持这样一种观点，即非产毒菌株的预先定殖可以改善与仔猪艰难梭菌感染相关的断奶前生长迟缓。
