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在过滤交配实验中，艰难梭菌 630Δerm 菌株中不存在毒素基因转移至非毒素产生艰难梭菌 NTCD-M3r 菌株。

Absence of toxin gene transfer from Clostridioides difficile strain 630Δerm to nontoxigenic C. difficile strain NTCD-M3r in filter mating experiments.

机构信息

Edward Hines, Jr. VA Hospital, Hines, Illinois, United States of America.

Loyola University Chicago Stritch School of Medicine, Maywood, Illinois, United States of America.

出版信息

PLoS One. 2022 Jun 29;17(6):e0270119. doi: 10.1371/journal.pone.0270119. eCollection 2022.

DOI:10.1371/journal.pone.0270119
PMID:35767545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9242483/
Abstract

Nontoxigenic Clostridioides difficile strain M3 (NTCD-M3) protects hamsters and humans against C. difficile infection. Transfer in vitro of the pathogenicity locus (PaLoc) to nontoxigenic strain CD37 has been reported. We repeated these conjugations using toxigenic strain 630Δerm as donor and NTCD-M3 and CD37 as recipients. In order to conduct these matings we induced rifampin resistance (50ug/ml) in NTCD-M3 by serial passage on rifampin-containing media to obtain strain NTCD-M3r. 630Δerm/CD37 matings produced 21 PaLoc transconjugants in 5.5 x 109 recipient CFUs; a frequency of 3.8 x 10-9. All transconjugants carried the tcdB gene and produced toxin. 630Δerm/NTCD-M3r matings produced no transconjugants in 5 assays with a total of 9.4 x 109 NTCD-M3r recipient cells. Toxin gene transfer to NTCD-M3r could not be demonstrated under conditions that demonstrated transfer to strain CD37.

摘要

无细胞毒素艰难梭状芽孢杆菌 M3（NTCD-M3）株可保护仓鼠和人类免受艰难梭菌感染。据报道，已将致病性基因座（PaLoc）通过体外转移到非细胞毒素的 CD37 株。我们使用产毒株 630Δerm 作为供体，NTCD-M3 和 CD37 作为受体重复了这些接合。为了进行这些交配，我们通过在含有利福平的培养基上连续传代使 NTCD-M3 产生利福平耐药性（50μg/ml），从而获得 NTCD-M3r 株。630Δerm/CD37 接合产生了 21 个 PaLoc 转导子，在 5.5×109 个受体 CFU 中，频率为 3.8×10-9。所有转导子均携带 tcdB 基因并产生毒素。在总共 9.4×109 个 NTCD-M3r 受体细胞的 5 次检测中，630Δerm/NTCD-M3r 接合未产生转导子。在已证明可向 CD37 株转移的条件下，未能证明毒素基因向 NTCD-M3r 的转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/acc8e355780a/pone.0270119.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/e4dfc20e5358/pone.0270119.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/381d02d71754/pone.0270119.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/acc8e355780a/pone.0270119.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/e4dfc20e5358/pone.0270119.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/381d02d71754/pone.0270119.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/404c/9242483/acc8e355780a/pone.0270119.g003.jpg

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The non-toxigenic strain of Clostridioides difficile Z31 can prevent infection by C. difficile in experimental model piglets.艰难梭菌非毒素株 Z31 可预防实验模型仔猪艰难梭菌感染。
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