Hol W H G, Van Veen A
Institute of Evolutionary and Ecological Sciences, Leiden University, The Netherlands.
J Chem Ecol. 2002 Sep;28(9):1763-72. doi: 10.1023/a:1020557000707.
We investigated the growth-reducing effects of pyrrolizidine alkaloids (PAs) from Senecio jacobaea on nine plant-associated fungi (five strains of Fusarium oxysporum, two of F. sambucinum, and two of Trichoderma sp). Fungal growth was monitored on water agar media containing different concentrations of monocrotaline, retrorsine, or a purified extract of PAs from S. jacobaea. The growth rate of six strains was inhibited by PAs at the highest test concentration (3.33 mM), with the magnitude of the inhibition (7-35%) being dependent upon the specific fungus-PA interaction. In general, the PA extract caused the largest inhibition. However, the fungi isolated from S. jacobaea were positively affected by the PA extract (7-9%). Retrorsine N oxide was as effective as retrorsine in its inhibition of mycelium growth.
我们研究了千里光中吡咯里西啶生物碱（PAs）对9种与植物相关真菌（5株尖孢镰刀菌、2株接骨木镰刀菌和2株木霉属真菌）生长的抑制作用。在含有不同浓度的野百合碱、倒千里光碱或千里光中PAs纯化提取物的水琼脂培养基上监测真菌生长。在最高测试浓度（3.33 mM）下，6株真菌的生长速率受到PAs抑制，抑制程度（7%-35%）取决于特定的真菌-PA相互作用。总体而言，PA提取物引起的抑制作用最大。然而，从千里光中分离出的真菌受到PA提取物的正向影响（7%-9%）。倒千里光碱N氧化物在抑制菌丝体生长方面与倒千里光碱效果相当。
