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[酵母比较遗传学。酿酒酵母的一个新的MEL15 α-半乳糖苷酶基因]

[Yeast comparative genetics. A new MEL15 alpha-galactosidase gene of Saccharomyces cerevisiae].

作者信息

Naumov G I, Naumova E S, Korshunova I V, Jakobsen M

机构信息

Institute of Genetics and Selection of Industrial Microorganisms, Russian Academy of Sciences, Moscow, 113545 Russia.

出版信息

Genetika. 2002 Oct;38(10):1330-6.

Abstract

To supplement the earlier identified European family of the highly homologous alpha-galactosidase MEL1-MEL11 genes and the African family of the divergent MEL12-MEL14 genes, a new MEL gene (MEL15) was found in several Saccharomyces cerevisiae strains isolated from maize dough in Ghana. Southern blotting and restriction enzyme analysis assigned the MEL15 gene to the African family and mapped it to chromosomes IV/XII, which migrate together in electrophoresis. Tetrad analysis ruled out the MEL15 location in the left arm of chromosome IV or the right arm of chromosome XII, which respectively contain the known MEL5 and MEL10 genes.

摘要

为补充早期鉴定出的高度同源的α-半乳糖苷酶MEL1 - MEL11基因的欧洲家族以及差异较大的MEL12 - MEL14基因的非洲家族,在从加纳玉米面团中分离出的几种酿酒酵母菌株中发现了一个新的MEL基因(MEL15)。Southern印迹法和限制性酶切分析将MEL15基因归为非洲家族,并将其定位到在电泳中一起迁移的IV/XII号染色体上。四分体分析排除了MEL15位于分别包含已知MEL5和MEL10基因的IV号染色体左臂或XII号染色体右臂上的可能性。

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