通过染色体印迹法和四分体分析将酿酒酵母的CDC3、CDC25和CDC42基因定位到第十二号染色体上。
Mapping of the Saccharomyces cerevisiae CDC3, CDC25, and CDC42 genes to chromosome XII by chromosome blotting and tetrad analysis.
作者信息
Johnson D I, Jacobs C W, Pringle J R, Robinson L C, Carle G F, Olson M V
机构信息
Department of Biology, University of Michigan, Ann Arbor 48109.
出版信息
Yeast. 1987 Dec;3(4):243-53. doi: 10.1002/yea.320030405.
CDC3, CDC25 and CDC42 were localized to chromosome XII by hybridizing the cloned genes to Southern blots of chromosomes separated by orthogonal-field-alternation gel electrophoresis. Meiotic tetrad analyses further localized these genes to the region distal to the RDN1 locus on the right arm of the chromosome. The STE11 gene, which had previously been mapped to chromosome XII (Chaleff and Tatchell, 1985), was found to be tightly linked to ILV5. The data suggest a map order of CEN12-RDN1-CDC42-(CDC25-CDC3)-(ILV5- STE11)-URA4. Certain oddities of the data set raise the possibility that there may be constraints on the patterns of recombination in this region of chromosome XII.
通过将克隆基因与经正交交变电场凝胶电泳分离的染色体Southern印迹杂交，发现CDC3、CDC25和CDC42定位于第十二号染色体上。减数分裂四分体分析进一步将这些基因定位到该染色体右臂上RDN1位点远端的区域。先前已定位到第十二号染色体上的STE11基因（Chaleff和Tatchell，1985年），被发现与ILV5紧密连锁。数据表明基因图谱顺序为CEN12-RDN1-CDC42-（CDC25-CDC3）-（ILV5-STE11）-URA4。该数据集的某些异常情况增加了第十二号染色体这一区域的重组模式可能受到限制的可能性。
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