鸡小肠分离细菌中胆汁盐水解酶活性的定量测定。
Quantitative determination of bile salt hydrolase activity in bacteria isolated from the small intestine of chickens.
作者信息
Knarreborg Ane, Engberg Ricarda M, Jensen Søren K, Jensen Bent B
机构信息
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, DK-8830 Tjele, Denmark.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6425-8. doi: 10.1128/AEM.68.12.6425-6428.2002.
Abstract
A quantitative assay based on high-performance liquid chromatography analysis of bile salts and bacterial protein determination was established for investigating bile salt hydrolase (BSH) activity in bacteria isolated from the small intestine of chickens. Bacteria were isolated using various media and were subsequently grouped according to cell morphology, fermentation profile, and 16S ribosomal DNA sequence. Representative isolates from each bacterial group were assayed for BSH activity. The isolates differed in BSH activity with respect to the state of growth and preculturing with and without taurochenodeoxycholate. The highest levels of BSH activity were found with Enterococcus faecium and Clostridium perfringens.
摘要
建立了一种基于胆汁盐高效液相色谱分析和细菌蛋白测定的定量分析方法，用于研究从鸡小肠分离出的细菌中的胆汁盐水解酶（BSH）活性。使用各种培养基分离细菌，随后根据细胞形态、发酵谱和16S核糖体DNA序列进行分组。对每个细菌组的代表性分离株进行BSH活性测定。分离株在生长状态以及有无牛磺鹅去氧胆酸盐预培养的情况下，其BSH活性有所不同。粪肠球菌和产气荚膜梭菌的BSH活性水平最高。
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本文引用的文献
1
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Appl Environ Microbiol. 1995 Jul;61(7):2577-82. doi: 10.1128/aem.61.7.2577-2582.1995.
2
Growth response to dietary penicillin of germ-free chicks and of chicks with a defined intestinal flora.无菌雏鸡和具有特定肠道菌群的雏鸡对日粮青霉素的生长反应。
Br J Nutr. 1959;13(1):78-84. doi: 10.1079/bjn19590012.
3
Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited.非培养法分析肠道细菌：重新审视猪胃肠道微生物群
Appl Environ Microbiol. 2002 Feb;68(2):673-90. doi: 10.1128/AEM.68.2.673-690.2002.
4
Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers.杆菌肽锌和盐霉素对肉鸡肠道微生物群及生产性能的影响。
Poult Sci. 2000 Sep;79(9):1311-9. doi: 10.1093/ps/79.9.1311.
5
Identification and characterization of a bile acid 7alpha-dehydroxylation operon in Clostridium sp. strain TO-931, a highly active 7alpha-dehydroxylating strain isolated from human feces.从人类粪便中分离出的高活性7α-脱羟基菌株梭菌属TO-931中胆汁酸7α-脱羟基操纵子的鉴定与表征
Appl Environ Microbiol. 2000 Mar;66(3):1107-13. doi: 10.1128/AEM.66.3.1107-1113.2000.
6
Evaluation of selective media for bifidobacteria in poultry and rabbit caecal samples.家禽和兔盲肠样本中双歧杆菌选择性培养基的评估
Zentralbl Veterinarmed B. 1999 Aug;46(6):369-73. doi: 10.1046/j.1439-0450.1999.00241.x.
7
Effect of Sodium Taurocholate on the In Vitro Growth of Lactobacilli.牛磺胆酸钠对乳酸杆菌体外生长的影响
Microb Ecol. 1997 Mar;33(2):163-7. doi: 10.1007/s002489900018.
8
Effect of dietary fiber on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs.膳食纤维对猪胃肠道不同区域微生物活性及微生物产气的影响。
Appl Environ Microbiol. 1994 Jun;60(6):1897-904. doi: 10.1128/aem.60.6.1897-1904.1994.
9
High concentrations of conjugated bile acids inhibit bacterial growth of Clostridium perfringens and induce its extracellular cholylglycine hydrolase.高浓度的结合胆汁酸可抑制产气荚膜梭菌的细菌生长，并诱导其细胞外胆酰甘氨酸水解酶的产生。
Steroids. 1994 Aug;59(8):485-9. doi: 10.1016/0039-128x(94)90062-0.
10
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