Zainuddin E N, Mundt S, Wegner U, Mentel R
Institute of Pharmacy, Department of Pharmaceutical Biology, Ernst-Moritz-Arndt University of Greifswald, Martin-Luther-Str. 6, 17487 Greifswald, Germany.
Med Microbiol Immunol. 2002 Dec;191(3-4):181-2. doi: 10.1007/s00430-002-0142-1. Epub 2002 Sep 5.
Aqueous and methanolic extracts of cultured cyanobacteria of several genera, Microcystis, Nodularia, Oscillatoria, Scytonema, Lyngbya and Calothrix were evaluated for their in vitro antiviral activity against influenza A virus in Madin Darby canine kidney cells. None of the methanolic extracts showed cytotoxic effects. The inhibitory concentration (IC(50)) of antiviral activity ranged between 20.0 micro g to 79.0 micro g extract/ml. The most active extract in this screening derived from genus Microcystis. The further analysis of methanolic extracts of cultured strains of genus Microcystis revealed a remarkable antiviral activity against influenza A virus for M. aeruginosa, M. ichthyoblabe and M. wesenbergii. The observed antiviral activity was associated with protease inhibitory activity of approximately 90% and suggest that protease inhibitory activity may be responsible for reducing virus replication. These results show that cyanobacteria are able to produce compounds with biological activity that may be of potential clinical interest.
对几个属的培养蓝藻细菌（微囊藻属、节球藻属、颤藻属、伪枝藻属、鞘丝藻属和眉藻属）的水提取物和甲醇提取物，在麦迪逊-达比犬肾细胞中针对甲型流感病毒的体外抗病毒活性进行了评估。甲醇提取物均未显示出细胞毒性作用。抗病毒活性的抑制浓度（IC50）在提取物20.0微克至79.0微克/毫升之间。此次筛选中最具活性的提取物来自微囊藻属。对微囊藻属培养菌株的甲醇提取物进行的进一步分析表明，铜绿微囊藻、鱼害微囊藻和惠氏微囊藻对甲型流感病毒具有显著的抗病毒活性。观察到的抗病毒活性与约90%的蛋白酶抑制活性相关，这表明蛋白酶抑制活性可能是减少病毒复制的原因。这些结果表明，蓝藻细菌能够产生具有生物活性的化合物，这些化合物可能具有潜在的临床意义。
