Turan K, Nagata K, Kuru A
Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Turkey.
Biochem Biophys Res Commun. 1996 Aug 5;225(1):22-6. doi: 10.1006/bbrc.1996.1125.
We have investigated the influence of Sanicula europaea L. extracts on influenza virus growth in MDCK cells. Fractions I, II, and III separated from Sanicula extract with Sephadex column chromatography were found to be non-toxic against MDCK cells. The growth of influenza A/PR/8/34 was completely inhibited by these fractions, while that of influenza B/Lee/40 was not affected. Fractions II and III have been shown not to have a direct virucidal activity on influenza A/PR/8/34. Influenza A/Vic/1/75 produced microscopic plaques in the presence of the extract. In vitro RNA synthesis with viral RNA-dependent RNA polymerase was also inhibited by a water soluble extract of Sanicula. These observations suggest that the Sanicula extract contains an anti-influenza virus substance.
我们研究了欧洲变豆菜提取物对MDCK细胞中流感病毒生长的影响。用葡聚糖凝胶柱色谱法从变豆菜提取物中分离出的组分I、II和III对MDCK细胞无毒。这些组分完全抑制了甲型流感病毒A/PR/8/34的生长，而对乙型流感病毒B/Lee/40的生长没有影响。已证明组分II和III对甲型流感病毒A/PR/8/34没有直接的杀病毒活性。在提取物存在的情况下，甲型流感病毒A/Vic/1/75产生了显微镜下可见的蚀斑。变豆菜的水溶性提取物也抑制了病毒RNA依赖性RNA聚合酶的体外RNA合成。这些观察结果表明，变豆菜提取物含有一种抗流感病毒物质。
