Kocsis Judit, Veres Amarilla, Vatay Agnes, Duba Jenö, Karádi István, Füst George, Prohászka Zoltán
3rd Department of Internal Medicine, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Immunol Invest. 2002 Aug-Nov;31(3-4):219-31. doi: 10.1081/imm-120016242.
Heat shock proteins (hsps) play complex role in the function of the immune system, they can activate both humoral and cellular immune response, as well the complement system. Although autoimmunity to hsp70 was implicated in certain autoimmune diseases and other conditions, the exact role of anti-hsp70 antibodies is not known. It was demonstrated by our previous work and other's findings that antibodies against the 60 kDa hsps are strongly associated with coronary atherosclerosis and carotis disease. It is also known that there is increased hsp70 expression at different sites of atherosclerosis. Therefore our aim was to study whether level of anti-hsp70 antibodies correlate with the presence of severe coronary artery disease (CAD). We measured and compared anti-hsp70 IgG antibody levels in CAD patients (n = 99) and healthy subjects (n = 99) with ELISA. The frequency of these antibodies was high in both groups and there was no significant difference in the median level of anti-hsp70 antibodies between patients with severe CAD and controls (653 (400-1141) vs. 630 (326-1152) AU/mL, P = 0.337). Adjustment for age, sex, BMI and lipid parameters did not change this result. Furthermore we did not find a correlation between anti-hsp70 antibody levels and certain risk factors of CAD (age, lipid parameters, body mass index, C-reactive protein, gender, smoking, diabetes and anti-hsp60 antibodies). By contrast, in accordance with our previous findings, anti-hsp60 and anti-hsp65 antibody levels were significantly higher in CAD patients, compared to this control group (p < 0.0001 for both variables). We did not find any correlation between the levels of anti-hsp70 and anti-hsp60 or anti-hsp65 antibodies either in the patients or the controls. The exact role of hsp70 in atherosclerosis is controversial, but we suggest that humoral immunity against human hsp70 does not contribute to coronary atherosclerosis in contrast to antibodies against 60kDa hsps.
热休克蛋白（hsps）在免疫系统功能中发挥着复杂的作用，它们既能激活体液免疫和细胞免疫反应，也能激活补体系统。尽管针对hsp70的自身免疫与某些自身免疫性疾病及其他病症有关，但抗hsp70抗体的确切作用尚不清楚。我们之前的研究工作以及其他研究结果表明，针对60 kDa热休克蛋白的抗体与冠状动脉粥样硬化和颈动脉疾病密切相关。另外还已知在动脉粥样硬化的不同部位hsp70表达增加。因此，我们的目的是研究抗hsp70抗体水平是否与严重冠状动脉疾病（CAD）的存在相关。我们采用酶联免疫吸附测定法（ELISA）测量并比较了CAD患者（n = 99）和健康受试者（n = 99）体内抗hsp70 IgG抗体水平。两组中这些抗体的出现频率都很高，严重CAD患者与对照组之间抗hsp70抗体的中位数水平无显著差异（653（400 - 1141）对630（326 - 1152）AU/mL，P = 0.337）。对年龄、性别、体重指数和血脂参数进行校正后，这一结果没有改变。此外，我们未发现抗hsp70抗体水平与CAD的某些危险因素（年龄、血脂参数、体重指数、C反应蛋白、性别、吸烟、糖尿病和抗hsp60抗体）之间存在相关性。相比之下，与我们之前的研究结果一致，CAD患者体内抗hsp60和抗hsp65抗体水平明显高于对照组（两个变量的p均< 0.0001）。无论是在患者组还是对照组中，我们都未发现抗hsp70与抗hsp60或抗hsp65抗体水平之间存在任何相关性。hsp70在动脉粥样硬化中的确切作用存在争议，但我们认为，与针对60 kDa热休克蛋白的抗体不同，针对人类hsp70的体液免疫不会导致冠状动脉粥样硬化。
