Nahas Eliana A P, Nahas-Neto Jorge, Orsatti Claudio L, Tardivo Ana Paula, Uemura Gilberto, Peraçoli Maria Terezinha S, Witkin Steven S
Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University-UNESP, Distrito de Rubião Júnior s/n, 18618-970, Botucatu, São Paulo, Brazil,
Cell Stress Chaperones. 2014 Jul;19(4):559-68. doi: 10.1007/s12192-013-0483-2. Epub 2013 Dec 11.
We investigated the association between circulating levels of 60 and 70 kDa heat-shock proteins (HSP60 and 70) and cardiovascular risk factors in postmenopausal women with or without metabolic syndrome (MetS). This cross-sectional study included 311 Brazilian women (age ≥45 years with amenorrhea ≥12 months). Women showing three or more of the following diagnostic criteria were diagnosed with MetS: waist circumference (WC) ≥88 cm, blood pressure ≥130/85 mmHg, triglycerides ≥150 mg/dl, high-density lipoprotein (HDL) <50 mg/dl, and glucose ≥100 mg/dl. Clinical, anthropometric, and biochemical parameters were collected. HSP60, HSP70, antibodies to HSP60 and HSP70, and C-reactive protein (CRP) levels were measured in serum. Student's t test, Kruskal-Wallis test, chi-square test, and Pearson correlation were used for statistical analysis. Of the 311 women, 30.9 % (96/311) were diagnosed with MetS. These women were, on average, obese with abdominal fat deposition and had lower HDL values as well as higher triglycerides and glucose levels. Homeostasis model assessment-insulin resistant (HOMA-IR) test values in these women were compatible with insulin resistance (P < 0.05). CRP and HSP60 concentrations were higher in women with MetS than in women without MetS (P < 0.05). HSP60, anti-HSP70, and CRP concentrations increased with the number of features indicative of MetS (P < 0.05). There was a significant positive correlation between anti-HSP70 and WC, blood pressure and HOMA-IR, and between CRP and WC, blood pressure, glucose, HOMA-IR, and triglycerides (P < 0.05). In postmenopausal women, serum HSP60 and anti-HSP70 concentrations increased with accumulating features of the metabolic syndrome. These results suggest a greater immune activation that is associated with cardiovascular risk in postmenopausal women with metabolic syndrome.
我们研究了60kDa和70kDa热休克蛋白（HSP60和HSP70）的循环水平与患有或未患有代谢综合征（MetS）的绝经后女性心血管危险因素之间的关联。这项横断面研究纳入了311名巴西女性（年龄≥45岁，闭经≥12个月）。出现以下三种或更多诊断标准的女性被诊断为患有MetS：腰围（WC）≥88厘米、血压≥130/85毫米汞柱、甘油三酯≥150毫克/分升、高密度脂蛋白（HDL）<50毫克/分升以及血糖≥100毫克/分升。收集了临床、人体测量和生化参数。测定了血清中的HSP60、HSP70、HSP60和HSP70抗体以及C反应蛋白（CRP）水平。采用学生t检验、Kruskal-Wallis检验、卡方检验和Pearson相关性分析进行统计分析。在311名女性中，30.9%（96/311）被诊断为患有MetS。这些女性平均肥胖且有腹部脂肪沉积，HDL值较低，甘油三酯和血糖水平较高。这些女性的稳态模型评估-胰岛素抵抗（HOMA-IR）测试值与胰岛素抵抗相符（P<0.05）。患有MetS的女性的CRP和HSP60浓度高于未患有MetS的女性（P<0.05）。HSP60、抗HSP70和CRP浓度随着表明MetS的特征数量增加而升高（P<0.05）。抗HSP70与WC、血压和HOMA-IR之间，以及CRP与WC、血压、血糖、HOMA-IR和甘油三酯之间存在显著正相关（P<0.05）。在绝经后女性中，血清HSP60和抗HSP70浓度随着代谢综合征特征的累积而升高。这些结果表明，在患有代谢综合征的绝经后女性中，免疫激活增强，这与心血管风险相关。
