Yang Miao, Wu Tangchun, Cheng Longxian, Wang Feng, Wei Qingyi, Tanguay Robert M
Institute of Occupational Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Respir Res. 2005 Feb 14;6(1):18. doi: 10.1186/1465-9921-6-18.
The heat shock proteins (Hsps) are induced by stresses such as allergic factors and inflammatory responses in bronchi epithelial cells and therefore may be detectable in patients with asthma. However, the etiologic link between anti-Hsps and asthma (its severity and related inflammatory responses such as interleukin-4 and immunoglobulin E) has not been established. We determined whether antibodies against Hsp60 and Hsp70 were present in patients with asthma and evaluated their associations with risk and severity of asthma.
We determined the levels of anti-Hsp60 and anti-Hsp70 by immunoblot and their associations with risk and symptom severity of asthma in 95 patients with asthma and 99 matched non-symptomatic controls using multivariate logistic regression analysis.
Compared to the controls, asthma patients were more likely to have detectable anti-Hsp60 (17.2% vs 5.1%) and anti-Hsp70 (33.7% vs 8.1%) (p < or = 0.001). In particular, the presence of anti-Hsp70 was associated with a greater than 2 fold risk for asthma (adjusted OR = 2.21; 95% CI = 1.35 approximately 3.59). Furthermore, both anti-Hsp60 and anti-Hsp70 levels were positively correlated with symptom severity (p < 0.05) as well as interleukin-4 and immunoglobulin E (p < 0.05). Individuals with antibodies against anti-Hsp60 and anti-Hsp70 were more likely to have a family history of asthma (p < 0.001) and higher plasma concentrations of total immunoglobulin E (p = 0.001) and interleukin-4 (p < 0.05) than those without antibodies.
These data suggest that anti-Hsp60 and especially anti-Hsp70 correlate with the attacks and severity of asthma. The underlying molecular mechanisms linking antibodies to heat shock proteins and asthma remain to be investigated.
热休克蛋白（Hsps）可由支气管上皮细胞中的过敏因素和炎症反应等应激诱导产生，因此在哮喘患者中可能可检测到。然而，抗热休克蛋白与哮喘（其严重程度以及白细胞介素 - 4和免疫球蛋白E等相关炎症反应）之间的病因学联系尚未确立。我们确定哮喘患者体内是否存在抗Hsp60和抗Hsp70抗体，并评估它们与哮喘风险和严重程度的关联。
我们通过免疫印迹法测定了95例哮喘患者和99例匹配的无症状对照者体内抗Hsp60和抗Hsp70的水平，并使用多因素逻辑回归分析评估了它们与哮喘风险和症状严重程度的关联。
与对照组相比，哮喘患者更有可能检测到抗Hsp60（17.2%对5.1%）和抗Hsp70（33.7%对8.1%）（p≤0.001）。特别是，抗Hsp70的存在与哮喘风险增加2倍以上相关（调整后的比值比 = 2.21；95%置信区间 = 1.35至3.59）。此外，抗Hsp60和抗Hsp70水平均与症状严重程度（p < 0.05）以及白细胞介素 - 4和免疫球蛋白E（p < 0.05）呈正相关。与没有抗体的个体相比，具有抗Hsp60和抗Hsp70抗体的个体更有可能有哮喘家族史（p < 0.001），并且总免疫球蛋白E（p = 0.001）和白细胞介素 - 4（p < 0.05）的血浆浓度更高。
这些数据表明抗Hsp60，尤其是抗Hsp70与哮喘的发作和严重程度相关。将抗体与热休克蛋白和哮喘联系起来的潜在分子机制仍有待研究。
