Forger J M, Choie D D, Friedberg E C
Cancer Res. 1976 Jan;36(1):258-62.
Chromatin proteins from control and dimethylnitrosamine-transformed baby hamster kidney cells were comparbd by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our results indicate that non-histone chromosomal proteins from transformed cells contained protein components of low and intermediate electrophoretic mobility, which were deficient in normal cells. Comparison of the relative amount of incorporation of labeled amino acids into non-histone chromosomal proteins showed that protein components with a molecular weight of about 60,000 M.W. had a markedly increased labeling activity in the chemically transformed cells. These results suggest that changes in non-histone chromosomal proteins are associated with neoplastic transformation by chemical carcinogens.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对对照及二甲基亚硝胺转化的幼仓鼠肾细胞的染色质蛋白进行了比较。我们的结果表明，转化细胞的非组蛋白染色体蛋白含有正常细胞中缺乏的低和中等电泳迁移率的蛋白成分。对标记氨基酸掺入非组蛋白染色体蛋白的相对量进行比较显示，分子量约为60,000的蛋白成分在化学转化细胞中的标记活性明显增加。这些结果表明，非组蛋白染色体蛋白的变化与化学致癌物引起的肿瘤转化有关。
