Gutierrez M Carmen, Kamekura Masahiro, Holmes Melissa L, Dyall-Smith Michael L, Ventosa Antonio
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Extremophiles. 2002 Dec;6(6):479-83. doi: 10.1007/s00792-002-0282-7. Epub 2002 Jul 30.
An extremely halophilic archaeon, previously named as Haloferax sp. strain Aa 2.2 or "Haloferax alicantei" that has been extensively used for genetic studies with halobacteria, was taxonomically characterized by using phenotypic tests (including morphological, physiological, biochemical and nutritional features), DNA-DNA hybridization and 16S rRNA sequence phylogenetic analysis. This organism was isolated in 1986 by Torreblanca et al. from a pond of a Spanish saltern located in Alicante. The cells were pleomorphic, Gram negative and grew optimally at 25% NaCl. The polar lipid composition was similar to that of species of the genus Haloferax. The DNA G+C content of this strain was 64.5 mol%. Phylogenetic analysis based on 16S rRNA sequence comparison confirmed that this archaeon is a member of the genus Haloferax and was most closely related to Haloferax volcanii. DNA-DNA hybridization between strain Aa 2.2 and the type strain of all named species of the genus Haloferax revealed low levels of relatedness (25-2%), supporting the placement of this organism in a new species. On the basis of the phenotypic characteristics, molecular data and phylogenetic analysis we propose to name strain Aa 2.2 as a new species, Haloferax lucentensis sp. nov. The type strain is Aa 2.2 (=JCM 9276=NCIMB 13854=CIP 107410=DSM 14919=CECT 5871=CCM 7023).
一种极端嗜盐古菌，先前命名为嗜盐富盐菌Aa 2.2菌株或“阿利坎特嗜盐富盐菌”，已被广泛用于嗜盐菌的遗传学研究，通过表型测试（包括形态、生理、生化和营养特征）、DNA-DNA杂交和16S rRNA序列系统发育分析对其进行了分类学特征鉴定。该菌株由托雷布兰卡等人于1986年从西班牙阿利坎特一处盐场的池塘中分离得到。细胞呈多形性，革兰氏阴性，在25%氯化钠浓度下生长最佳。极性脂质组成与嗜盐富盐菌属的物种相似。该菌株的DNA G+C含量为64.5摩尔%。基于16S rRNA序列比较的系统发育分析证实，这种古菌是嗜盐富盐菌属的成员，与沃氏嗜盐富盐菌关系最为密切。Aa 2.2菌株与嗜盐富盐菌属所有已命名物种的模式菌株之间的DNA-DNA杂交显示出较低的相关性（25%-2%），支持将该菌株归为一个新物种。基于表型特征、分子数据和系统发育分析，我们提议将Aa 2.2菌株命名为一个新物种，即明亮嗜盐富盐菌（Haloferax lucentensis sp. nov.）。模式菌株为Aa 2.2（=JCM 9276=NCIMB 13854=CIP 107410=DSM 14919=CECT 5871=CCM 7023）。
