Narui Koji, Noguchi Norihisa, Wakasugi Kazunori, Sasatsu Masanori
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Biol Pharm Bull. 2002 Dec;25(12):1533-6. doi: 10.1248/bpb.25.1533.
A novel drug efflux gene (named sepA, staphylococcal efflux pump gene) is cloned from antiseptic-resistant mutants of Staphylococcus aureus into Escherichia coli. The sepA gene conferred the reduction of susceptibility to acriflavine and the acceleration of ethidium bromide efflux from the E. coli cells. The sepA (474 bp) encoded the protein that has four predicted transmembrane segments. These results indicate that sepA gene is a multidrug-resistant gene and encodes a drug efflux protein.
从金黄色葡萄球菌的抗防腐剂突变体中克隆出一个新的药物外排基因（命名为sepA，即葡萄球菌外排泵基因），并将其导入大肠杆菌。sepA基因使大肠杆菌对吖啶黄素的敏感性降低，并加速溴化乙锭从大肠杆菌细胞中的外排。sepA（474 bp）编码的蛋白质有四个预测的跨膜区段。这些结果表明，sepA基因是一个多药耐药基因，编码一种药物外排蛋白。
