Yamada Youichi, Hideka Koh-ichiro, Shiota Sumiko, Kuroda Teruo, Tsuchiya Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
Biol Pharm Bull. 2006 Mar;29(3):554-6. doi: 10.1248/bpb.29.554.
There are more than 30 genes for putative multidrug efflux pumps in the chromosome of Staphylococcus aureus. Only a few of these have been analyzed so far. Here we cloned a new gene, SA1972, using a PCR method, from the chromosome of S. aureus N315. We found that the product SA1972 could lead to elevated resistance against several antimicrobial agents such as norfloxacin, acriflavine and ethidium bromide. We designated the gene as sdrM. We observed elevated energy-dependent efflux of acriflavine in S. aureus cells introduced with the sdrM gene. We conclude that SdrM is a multidrug efflux pump belonging to the major facilitator (MF) superfamily.
金黄色葡萄球菌染色体上有30多个假定的多药外排泵基因。到目前为止，仅对其中少数基因进行了分析。在此，我们使用PCR方法从金黄色葡萄球菌N315染色体中克隆了一个新基因SA1972。我们发现SA1972产物可导致对几种抗菌剂（如诺氟沙星、吖啶黄素和溴化乙锭）的耐药性升高。我们将该基因命名为sdrM。我们观察到导入sdrM基因的金黄色葡萄球菌细胞中吖啶黄素的能量依赖性外排增加。我们得出结论，SdrM是一种属于主要转运体（MF）超家族的多药外排泵。
