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染色质模型计算：球形核小体阵列

Chromatin model calculations: Arrays of spherical nu bodies.

作者信息

Carlson R D, Olins D E

出版信息

Nucleic Acids Res. 1976 Jan;3(1):89-100. doi: 10.1093/nar/3.1.89.

DOI:10.1093/nar/3.1.89
PMID:1250708
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342880/
Abstract

Chromatin fibers consists of globular nucleohistone particles (designated nu bodies) along the length of the chromatin DNA with approximately 6-to7-fold compaction of the DNA within the nu bodies. We have calculated theoretical small-angle x-ray scattering curves and have compared these with experimental data in the literature. Several models predict maxima at the correct angles. The first maximum (approximately 110 degrees A) results from interparticle interference, while both the spatial arrangement and the structure factor the nu bodies can contribute to the additional small-angle maxima. These calculations suggest models which can account for the electron microscopic observation that chromatin is seen as either approximately 100-or approximately 200-to 250 degrees A-diameter fibers, depending on the solvent conditions. They also account for the limited orientability of the x-ray pattern from pulled chromatin fibers.

摘要

染色质纤维由沿染色质DNA长度分布的球状核组蛋白颗粒（称为核小体）组成，核小体内的DNA压缩了约6至7倍。我们计算了理论小角X射线散射曲线，并将其与文献中的实验数据进行了比较。几个模型在正确的角度预测到了最大值。第一个最大值（约110埃）来自粒子间的干涉，而核小体的空间排列和结构因子都可能导致额外的小角最大值。这些计算提出的模型能够解释电子显微镜观察结果，即根据溶剂条件，染色质可呈现为直径约100埃或约200至250埃的纤维。它们还解释了拉伸染色质纤维的X射线图案的有限取向性。

相似文献

1
Chromatin model calculations: Arrays of spherical nu bodies.染色质模型计算：球形核小体阵列
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2
Visualization of chromatin substructure: upsilon bodies.染色质亚结构的可视化：υ小体。
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3
DNA chain flexibility and the structure of chromatin nu-bodies.DNA链的柔韧性与染色质核小体的结构
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Model studies of chromatin structure based on X-ray diffraction data.基于X射线衍射数据的染色质结构模型研究。
Nucleic Acids Res. 1976 Nov;3(11):3025-42. doi: 10.1093/nar/3.11.3025.
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Urea denaturation of chromatin periodic structure.染色质周期性结构的尿素变性
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Organisation of subunits in chromatin.染色质中亚基的组织
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The superstructure of chromatin and its condensation mechanism. II. Theoretical analysis of the X-ray scattering patterns and model calculations.染色质的上层结构及其凝聚机制。II. X射线散射图谱的理论分析与模型计算。
Eur Biophys J. 1986;13(3):175-85. doi: 10.1007/BF00542561.
8
[Folding of deoxyribonucleic acid in chromatin].[染色质中脱氧核糖核酸的折叠]
Acta Biol Med Ger. 1975;34(11-12):K47-56.
9
Chromatin nu bodies: isolation, subfractionation and physical characterization.染色质核小体：分离、亚分级分离及物理特性分析
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10
Chromatin fibers are left-handed double helices with diameter and mass per unit length that depend on linker length.染色质纤维是左手双螺旋结构，其直径和单位长度质量取决于连接体长度。
Biophys J. 1986 Jan;49(1):233-48. doi: 10.1016/S0006-3495(86)83637-2.

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Differences in nanoscale organization of regulatory active and inactive human chromatin.人类染色质调控活性与非活性状态下纳米尺度组织的差异。
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2
Interparticle effects in low-angle x-ray and neutron diffraction from chromatin.染色质低角度X射线和中子衍射中的颗粒间效应。
Biophys J. 1980 May;30(2):307-16. doi: 10.1016/S0006-3495(80)85096-X.
3
Solenoidal model for superstructure in chromatin.染色质上层结构的螺线管模型。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901. doi: 10.1073/pnas.73.6.1897.
4
Chromatin nu bodies: isolation, subfractionation and physical characterization.染色质核小体：分离、亚分级分离及物理特性分析
Nucleic Acids Res. 1976 Dec;3(12):3271-91. doi: 10.1093/nar/3.12.3271.
5
Model studies of chromatin structure based on X-ray diffraction data.基于X射线衍射数据的染色质结构模型研究。
Nucleic Acids Res. 1976 Nov;3(11):3025-42. doi: 10.1093/nar/3.11.3025.
6
Organisation of subunits in chromatin.染色质中亚基的组织
Nucleic Acids Res. 1976 Jul;3(7):1739-46. doi: 10.1093/nar/3.7.1739.
7
Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions.染色质小角中子散射：稀溶液中30纳米直径超螺旋的进一步证据。
Nucleic Acids Res. 1977 Jul;4(7):2275-82. doi: 10.1093/nar/4.7.2275.
8
Chromatin fiber dimensions and nucleosome orientation: a neutron scattering investigation.染色质纤维尺寸与核小体取向：中子散射研究
Nucleic Acids Res. 1978 Oct;5(10):3697-714. doi: 10.1093/nar/5.10.3697.
9
Stereo electron microscopy of the 25-nm chromatin fibers in isolated nuclei.分离细胞核中25纳米染色质纤维的立体电子显微镜观察。
J Cell Biol. 1979 Apr;81(1):260-5. doi: 10.1083/jcb.81.1.260.
10
Nucleosome packing in interphase chromatin.间期染色质中的核小体组装
J Cell Biol. 1979 May;81(2):453-7. doi: 10.1083/jcb.81.2.453.

本文引用的文献

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Chromatin structure: deduced from a minichromosome.染色质结构：从小染色体推断出来的。
Science. 1975 Mar 28;187(4182):1202-3. doi: 10.1126/science.187.4182.1202.
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Physical studies of the molecular structure of deoxyribose nucleic acid and nucleoprotein.脱氧核糖核酸和核蛋白分子结构的物理学研究。
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A model for particulate structure in chromatin.染色质中颗粒结构的模型。
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The relationship of isolated chromosome fibers to the fibers of the embedded nucleus.分离的染色体纤维与嵌入细胞核的纤维之间的关系。
J Ultrastruct Res. 1967 Aug;19(3):382-97. doi: 10.1016/s0022-5320(67)80226-0.
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Super-helical model for nucleohistone.核组蛋白的超螺旋模型
Nature. 1967 Jul 29;215(5100):508-9. doi: 10.1038/215508a0.
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Macromolecular organization of nuclei and chromosomes: a folded fibre model based on whole-mount electron microscopy.细胞核与染色体的大分子组织：基于整装电子显微镜的折叠纤维模型
Nature. 1965 Apr 24;206(982):338-43. doi: 10.1038/206338a0.
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Electron-microscope observations on the organization of heterochromatin in certain cells.关于某些细胞中异染色质组织的电子显微镜观察
J Cell Sci. 1968 Mar;3(1):129-50. doi: 10.1242/jcs.3.1.129.
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Effects of low salt concentration on structural organization and template activity of chromatin in chicken erythrocyte nuclei.低盐浓度对鸡红细胞核染色质结构组织和模板活性的影响。
Exp Cell Res. 1971 Mar;65(1):61-72. doi: 10.1016/s0014-4827(71)80050-2.
10
The molecular structure of nucleohistone (DNH).核组蛋白（DNH）的分子结构。
Exp Cell Res. 1970 Sep;62(1):184-96. doi: 10.1016/0014-4827(79)90519-6.

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