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染色质模型计算：球形核小体阵列

Chromatin model calculations: Arrays of spherical nu bodies.

作者信息

Carlson R D, Olins D E

出版信息

Nucleic Acids Res. 1976 Jan;3(1):89-100. doi: 10.1093/nar/3.1.89.

DOI:10.1093/nar/3.1.89
PMID:1250708
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342880/
Abstract

Chromatin fibers consists of globular nucleohistone particles (designated nu bodies) along the length of the chromatin DNA with approximately 6-to7-fold compaction of the DNA within the nu bodies. We have calculated theoretical small-angle x-ray scattering curves and have compared these with experimental data in the literature. Several models predict maxima at the correct angles. The first maximum (approximately 110 degrees A) results from interparticle interference, while both the spatial arrangement and the structure factor the nu bodies can contribute to the additional small-angle maxima. These calculations suggest models which can account for the electron microscopic observation that chromatin is seen as either approximately 100-or approximately 200-to 250 degrees A-diameter fibers, depending on the solvent conditions. They also account for the limited orientability of the x-ray pattern from pulled chromatin fibers.

摘要

染色质纤维由沿染色质DNA长度分布的球状核组蛋白颗粒（称为核小体）组成，核小体内的DNA压缩了约6至7倍。我们计算了理论小角X射线散射曲线，并将其与文献中的实验数据进行了比较。几个模型在正确的角度预测到了最大值。第一个最大值（约110埃）来自粒子间的干涉，而核小体的空间排列和结构因子都可能导致额外的小角最大值。这些计算提出的模型能够解释电子显微镜观察结果，即根据溶剂条件，染色质可呈现为直径约100埃或约200至250埃的纤维。它们还解释了拉伸染色质纤维的X射线图案的有限取向性。

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