Papoulias Diana M, Villalobos Sergio A, Meadows John, Noltie Douglas B, Giesy John P, Tillitt Donald E
U.S. Geological Survey, Columbia Environmental Research Center, Columbia, Missouri 65201, USA.
Environ Health Perspect. 2003 Jan;111(1):29-32. doi: 10.1289/ehp.5540.
Despite being banned in many countries, dichlorodiphenyltrichloroethane (DDT) and its metabolites dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD) continue to be found in fish tissues at concentrations of concern. Like o,p -DDT, o,p -DDE is estrogenic and is believed to exert its effects through binding to the estrogen receptor. The limited toxicologic data for o,p -DDE suggest that it decreases fecundity and fertility of fishes. We conducted an egg injection study using the d-rR strain of medaka and environmentally relevant concentrations of o,p -DDE to examine its effects on sexual differentiation and development. The gonads of exposed fish showed no evidence of sex reversal or intersex. However, other gonad abnormalities occurred in exposed individuals. Females exhibited few vitellogenic oocytes and increased atresia. Male testes appeared morphologically normal but were very small. Gonadosomatic index values for both sexes were lower for exposed fish. Our observations of abnormal female and very small male gonads after in ovo o,p -DDE exposure may be indicative of effects on early endocrine processes important for normal ovarian and testicular development.
尽管在许多国家已被禁用，但在鱼类组织中仍能发现浓度令人担忧的二氯二苯三氯乙烷（DDT）及其代谢产物二氯二苯二氯乙烯（DDE）和二氯二苯二氯乙烷（DDD）。与邻，对-DDT一样，邻，对-DDE具有雌激素活性，据信它通过与雌激素受体结合发挥作用。关于邻，对-DDE的有限毒理学数据表明，它会降低鱼类的繁殖力和生育能力。我们使用青鳉的d-rR品系和与环境相关浓度的邻，对-DDE进行了一项卵注射研究，以检验其对性别分化和发育的影响。暴露鱼的性腺没有出现性逆转或雌雄同体的迹象。然而，暴露个体出现了其他性腺异常。雌性的卵黄生成卵母细胞很少且闭锁增加。雄性睾丸形态上看似正常，但非常小。暴露鱼的两性性体指数值都较低。我们观察到在卵内暴露于邻，对-DDE后雌性性腺异常且雄性性腺非常小，这可能表明其对正常卵巢和睾丸发育重要的早期内分泌过程有影响。
