Stuppy Wolfgang H, Maisano Jessica A, Colbert Matthew W, Rudall Paula J, Rowe Timothy B
Royal Botanic Gardens, Kew, Seed Conservation Department, Wakehurst Place, Ardingly, West Sussex RH7 6TN, UK.
Trends Plant Sci. 2003 Jan;8(1):2-6. doi: 10.1016/s1360-1385(02)00004-3.
High-resolution X-ray computed tomography (HRCT) is a non-invasive approach to 3D visualization and quantification of biological structure. The data, based on differential X-ray attenuation, are analogous to those otherwise obtainable only by serial sectioning. Requiring no fixing, sectioning or staining, HRCT produces a 3D digital map of the specimen that allows measurements and visualizations, including arbitrarily oriented sections. In spite of its application throughout the natural sciences, HRCT has yet to be applied in extant plant structural research.
高分辨率X射线计算机断层扫描(HRCT)是一种用于生物结构三维可视化和定量分析的非侵入性方法。基于X射线衰减差异的数据类似于那些只能通过连续切片才能获得的数据。HRCT无需固定、切片或染色,就能生成标本的三维数字地图,可进行测量和可视化,包括任意方向的切片。尽管HRCT已在整个自然科学领域得到应用,但尚未应用于现存的植物结构研究中。