Arn Eric A, Cha Byeong J, Theurkauf William E, Macdonald Paul M
Institute for Cellular and Molecular Biology, Section of Molecular Cell and Developmental Biology, University of Texas, Austin, TX 78712, USA.
Dev Cell. 2003 Jan;4(1):41-51. doi: 10.1016/s1534-5807(02)00397-0.
Localization of mRNAs, a process essential for embryonic body patterning in Drosophila, requires recognition of cis-acting signals by cellular components responsible for movement and anchoring. We have purified a large multiprotein complex that binds a minimal form of the bicoid mRNA localization signal in a manner both specific and sensitive to inactivating mutations. Identified complex components include the RNA binding proteins Modulo, PABP, and Smooth, the known localization factor Swallow, and the kinesin family member Nod. We demonstrate that localization of bcd mRNA is defective in modulo mutants. The presence of three required localization components (Swallow, Modulo, and specific RNA binding activity) within the recognition complex strongly implicates it in mRNA localization.
mRNA的定位是果蝇胚胎体模式形成所必需的过程，它需要负责移动和锚定的细胞成分识别顺式作用信号。我们纯化了一种大型多蛋白复合物，该复合物以对失活突变既特异又敏感的方式结合双尾mRNA定位信号的最小形式。鉴定出的复合物成分包括RNA结合蛋白Modulo、PABP和平滑蛋白，已知的定位因子吞咽蛋白，以及驱动蛋白家族成员Nod。我们证明，在modulo突变体中，bcd mRNA的定位存在缺陷。识别复合物中存在三种必需的定位成分（吞咽蛋白、Modulo和特定的RNA结合活性），这强烈表明它参与了mRNA的定位。
