Karjalainen J, Hulkkonen J, Nieminen M M, Huhtala H, Aromaa A, Klaukka T, Hurme M
Department of Respiratory Medicine, Tampere University Hospital, Tampere, Finland.
Clin Exp Allergy. 2003 Jan;33(1):78-83. doi: 10.1046/j.1365-2222.2003.01577.x.
IL-10 has several functional effects relevant to asthma. It can modulate IgE production and induce apoptosis in eosinophils. Polymorphisms of IL-10 gene have been shown to affect IL-10 production.
To establish whether IL-10 polymorphisms are associated with asthma and phenotype-related characteristics.
The frequency of three single base exchange polymorphisms (at positions - 1082, - 819 and - 592) and corresponding haplotypes of the IL-10 gene were analysed in 245 adult asthmatic subjects and 405 controls using PCR and restriction fragment length polymorphism (RFLP). The data were assessed for correlations with the eosinophil count, serum IgE and lung function.
The IL-10 haplotype frequencies were similar in asthmatics and controls. Eosinophil count median was 2.0- to 3.2-fold higher among asthmatics with rare ATA/ATA genotype than in asthmatics with other genotypes. No such difference was seen in the control group. When analysed by IL-10 haplotype carrier state and gender, male asthmatics with ATA haplotype had 2.8-fold higher serum IgE than those without ATA. A converse association was found in male controls with ATA haplotype, who had 1.9-fold lower serum IgE than their ATA-negative counterparts. The high IL-10-producing GCC haplotype was associated with impaired lung function in smoking male controls while in asthmatics no clear effect on lung function was found with any of the haplotypes studied.
These results suggest that the eosinophil counts and serum IgE are differently regulated by IL-10 genotype in asthmatic and in normal subjects. However, IL-10 polymorphism is not related to susceptibility in asthma.
白细胞介素-10（IL-10）具有多种与哮喘相关的功能效应。它可调节免疫球蛋白E（IgE）的产生并诱导嗜酸性粒细胞凋亡。白细胞介素-10基因多态性已被证明会影响IL-10的产生。
确定IL-10基因多态性是否与哮喘及表型相关特征有关。
采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析了245名成年哮喘患者和405名对照者中白细胞介素-10基因的三个单碱基交换多态性（位于-1082、-819和-592位点）及相应单倍型。评估这些数据与嗜酸性粒细胞计数、血清IgE和肺功能的相关性。
哮喘患者和对照者中IL-10单倍型频率相似。具有罕见ATA/ATA基因型的哮喘患者的嗜酸性粒细胞计数中位数比具有其他基因型的哮喘患者高2.0至3.2倍。对照组未观察到此类差异。按IL-10单倍型携带状态和性别分析时，具有ATA单倍型的男性哮喘患者的血清IgE比没有ATA单倍型的患者高2.8倍。在具有ATA单倍型的男性对照者中发现了相反的关联，他们的血清IgE比ATA阴性的对照者低1.9倍。产生高IL-10的GCC单倍型与吸烟男性对照者的肺功能受损有关，而在哮喘患者中，所研究的任何单倍型对肺功能均未发现明显影响。
这些结果表明，哮喘患者和正常受试者中嗜酸性粒细胞计数和血清IgE受IL-10基因型的调节方式不同。然而，IL-10基因多态性与哮喘易感性无关。
