Li Q, Jiang L, Zan Y, Zhao H, Guo R
Institute of Medical Biology, CAMS, PUMC, Kunming 650107.
Wei Sheng Wu Xue Bao. 1998 Apr;38(2):86-91.
The construction of poliovirus chimera is an useful way to investigate the antigenic structure conformation of poliovirus. In this research, the poliovirus chimera containing a piece of antigenic fragment of hepatitis A virus was constructed for analysis of poliovirus antigenic structure conformation. Depending on the characterization of this chimera, the conformation of poliovirus neutralization antigenic site I in VP1 was analyzed, and that the possible influence of hepatitis A virus antigenic fragment inserted to the structure conformation of poliovirus antigen was also investigated.
构建脊髓灰质炎病毒嵌合体是研究脊髓灰质炎病毒抗原结构构象的一种有效方法。在本研究中，构建了含有甲型肝炎病毒一段抗原片段的脊髓灰质炎病毒嵌合体，用于分析脊髓灰质炎病毒的抗原结构构象。根据该嵌合体的特性，分析了VP1中脊髓灰质炎病毒中和抗原位点I的构象，并研究了插入的甲型肝炎病毒抗原片段对脊髓灰质炎病毒抗原结构构象可能产生的影响。
