Hormbrey E, Gillespie P, Turner K, Han C, Roberts A, McGrouther D, Harris A L
Weatherall Institute of Molecular Medicine, Headington, Oxford, UK.
Clin Exp Metastasis. 2002;19(8):651-63. doi: 10.1023/a:1021379811308.
Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor with a key role in many physiological and pathological processes. Investigation into the implications of circulating levels of this cytokine is progressing at an exponential rate. However, there are important inconsistencies between reports ranging from method of sample collection, processing, software manipulation and data interpretation and controversy as to whether plasma, serum or whole blood will provide the best prognostic information. Different techniques of centrifugation and temperature on sample handling and the impact of in vitro collection of blood on subsequent VEGF results have not been fully appreciated. We provide a critical review of the literature, report the results of our further investigations, suggest a uniform protocol for handling blood samples and highlight previously unsuspected problems in data interpretation.
血管内皮生长因子（VEGF）是一种强效血管生成生长因子，在许多生理和病理过程中起关键作用。对这种细胞因子循环水平影响的研究正在以指数级速度推进。然而，从样本采集方法、处理、软件操作和数据解释等方面来看，不同报告之间存在重要的不一致之处，并且对于血浆、血清或全血是否能提供最佳预后信息也存在争议。不同的离心技术和温度对样本处理的影响以及体外采血对后续VEGF结果的影响尚未得到充分认识。我们对文献进行了批判性综述，报告了我们进一步研究的结果，提出了处理血样的统一方案，并强调了数据解释中先前未被怀疑的问题。
