Ouédraogo J B, Dutheil Y, Tinto H, Traoré B, Zampa H, Tall F, Coulibaly S O, Guiguemdé T R
Centre Muraz/OCCGE, Malaria Chemoresistance Reference Centre, Parasitology and Entomology Laboratory Bobo-Dioulasso, Burkina Faso.
Trop Med Int Health. 1998 May;3(5):381-4.
The in vitro sensitivity of P. falciparum drug-resistant isolates was evaluated in the region of Bobo-Dioulasso during the 1995 and 1996 rainy seasons. Two routinely used antimalarials (chloroquine and quinine) and two new antimalarials (mefloquine and halofantrine) were assessed using 24-hour in vitro cultures with tritiated hypoxanthine and a parasite density > or = 4,000/microl of blood. The proportion of chloroquine-resistant isolates was 20% in 1995 and 19% in 1996, whilst in 1996, the proportion of isolates resistant to halofantrine was greater than in 1995 (9.6% versus 1%). No significant differences were seen in the mean IC50 values in relation to the susceptibility of chloroquine-resistant or chloroquine-sensitive isolates to mefloquine and halofantrine. In the case of quinine, the mean IC50 values were significantly higher in chloroquine-resistant isolates than in chloroquine-sensitive ones. A significant positive correlation was found between the following IC50 values: chloroquine versus quinine, quinine versus mefloquine and mefloquine versus halofantrine.
1995年和1996年雨季期间，在博博迪乌拉索地区评估了恶性疟原虫耐药分离株的体外敏感性。使用含氚次黄嘌呤的24小时体外培养物和寄生虫密度≥4000/微升血液，对两种常规使用的抗疟药（氯喹和奎宁）以及两种新型抗疟药（甲氟喹和卤泛群）进行了评估。1995年氯喹耐药分离株的比例为20%，1996年为19%，而在1996年，卤泛群耐药分离株的比例高于1995年（9.6%对1%）。氯喹耐药或氯喹敏感分离株对甲氟喹和卤泛群的敏感性方面，平均半数抑制浓度（IC50）值未见显著差异。就奎宁而言，氯喹耐药分离株的平均IC50值显著高于氯喹敏感分离株。在以下IC50值之间发现显著正相关：氯喹与奎宁、奎宁与甲氟喹以及甲氟喹与卤泛群。
