Stock Ingo, Falsen Enevold, Wiedemann Bernd
Institute of Medical Microbiology and Immunology, Pharmaceutical Microbiology, University of Bonn, Bonn, Germany.
Diagn Microbiol Infect Dis. 2003 Jan;45(1):1-11. doi: 10.1016/s0732-8893(02)00483-2.
The present study establishes a data compilation on biochemical features and natural antibiotic susceptibilities of Moellerella wisconsensis strains. 17 moellerellae isolated from humans (n = 11), food (n = 5) and water (n = 1) were tested. Identification was carried out using two commercially available systems and conventional tests. MIC determinations of 74 antibiotics were performed applying a microdilution procedure in Cation-adjusted Mueller Hinton broth and IsoSensitest broth. M. wisconsensis was naturally sensitive to doxycycline, minocycline, all tested aminoglycosides, numerous beta-lactams, all fluoroquinolones, folate-pathway inhibitors, chloramphenicol and nitrofurantoin. Natural resistance was found with oxacillin, penicillin G, all tested macrolides, lincomycin, streptogramins, ketolides, glycopeptides, fusidic acid, linezolid and rifampicin. Medium-dependent differences in susceptibility affecting clinical assessment criteria were seen with tetracycline, clindamycin and fosfomycin. From the data of the present study it is possible that some moellerellae are misidentified as Klebsiella pneumoniae subsp. ozaenae.
本研究建立了威斯康星莫氏杆菌菌株生化特征和天然抗生素敏感性的数据汇编。对从人类（n = 11）、食物（n = 5）和水（n = 1）中分离出的17株莫氏杆菌进行了检测。使用两种市售系统和常规试验进行鉴定。在阳离子调节的穆勒-欣顿肉汤和异感测肉汤中采用微量稀释法测定了74种抗生素的最低抑菌浓度（MIC）。威斯康星莫氏杆菌对多西环素、米诺环素、所有检测的氨基糖苷类、多种β-内酰胺类、所有氟喹诺酮类、叶酸途径抑制剂、氯霉素和呋喃妥因天然敏感。对苯唑西林、青霉素G、所有检测的大环内酯类、林可霉素、链阳菌素、酮内酯类、糖肽类、夫西地酸、利奈唑胺和利福平天然耐药。四环素、克林霉素和磷霉素存在影响临床评估标准的药敏的培养基依赖性差异。根据本研究的数据，有可能一些莫氏杆菌被误鉴定为肺炎克雷伯菌亚种奥泽那菌。
