Hamberg H, Brunk U T, Ericsson J L, Jung B
Acta Pathol Microbiol Scand A. 1976 Mar;84(2):201-14. doi: 10.1111/j.1699-0463.1976.tb00090.x.
The investigation was initiated with the aim of establishing a suitable experimental model with respect to mode of radiation and radiation dose for elucidating morphologically the sequential development of radiation induced damage of interphase cells (human glia cells in vitro). Adequately defined and reproducible cellular changes were obtained using X-radiation generated by an 8 MeV linear accelerator at a dose of 20,000 rad. The cellular alterations were studied in the light microscope and by scanning and transmission electron microscopy. The most conspicuous changes--first appreciable about 5 hours after irradiation--occurred in the lysosomal vacuome, and the plasma membrane and associated structures.
这项研究的目的是建立一个关于辐射方式和辐射剂量的合适实验模型，以便从形态学上阐明间期细胞（体外培养的人神经胶质细胞）辐射诱导损伤的连续发展过程。使用一台8兆电子伏特直线加速器产生的X射线，以20000拉德的剂量进行照射，获得了充分明确且可重复的细胞变化。通过光学显微镜、扫描电子显微镜和透射电子显微镜对细胞改变进行了研究。最明显的变化——照射后约5小时首次明显出现——发生在溶酶体泡状系统、质膜及相关结构中。
