Zeina B, Greenman J, Corry D, Purcell W M
Dermatology Department of Teshreen Hospital, Damascus, Syria.
Br J Dermatol. 2003 Feb;148(2):229-32. doi: 10.1046/j.1365-2133.2003.05091.x.
Work has shown that cutaneous microbial species associated with skin conditions of microbial aetiology are susceptible to killing by antimicrobial photodynamic therapy (APDT) using visible light and methylene blue.
To evaluate immediate and delayed genotoxicity of APDT on keratinocytes in vitro.
A combination of methylene blue (100 microg mL(-1)) and visible light (42 mW cm(-2)), as used in studies of microbe and keratinocyte cytotoxicity, was employed to test a human keratinocyte cell line (H103) for genotoxic damage by comet assay.
The comet assay was able to detect genotoxic damage in H2O2-treated keratinocytes (positive control). APDT did not cause either immediate or delayed genotoxic damage in keratinocytes following APDT of up to 180 min.
APDT sufficient to reduce microbes by seven log cycles showed no detectable genotoxic effects on keratinocytes. APDT applied in vivo may represent a useful low-risk alternative to conventional antimicrobial treatment in dermatology.
研究表明，与微生物病因引起的皮肤疾病相关的皮肤微生物种类，对使用可见光和亚甲蓝的抗菌光动力疗法（APDT）敏感，可被其杀灭。
评估APDT在体外对角质形成细胞的即时和延迟遗传毒性。
采用微生物和角质形成细胞细胞毒性研究中使用的亚甲蓝（100μg mL⁻¹）和可见光（42 mW cm⁻²）组合，通过彗星试验检测人角质形成细胞系（H103）的遗传毒性损伤。
彗星试验能够检测到过氧化氢处理的角质形成细胞（阳性对照）中的遗传毒性损伤。在长达180分钟的APDT后，APDT对角质形成细胞既没有造成即时的也没有造成延迟的遗传毒性损伤。
足以使微生物数量减少7个对数周期的APDT，对角质形成细胞未显示出可检测到的遗传毒性作用。体内应用APDT可能是皮肤科传统抗菌治疗的一种有用的低风险替代方法。
