Yadav R S, Bidinger F R, Hash C T, Yadav Y P, Yadav O P, Bhatnagar S K, Howarth C J
Institute of Grassland and Environmental Research (IGER), Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB Wales, UK.
Theor Appl Genet. 2003 Feb;106(3):512-20. doi: 10.1007/s00122-002-1081-3. Epub 2002 Sep 13.
A mapping population of 104 F(3) lines of pearl millet, derived from a cross between two inbred lines H 77/833-2 x PRLT 2/89-33, was evaluated, as testcrosses on a common tester, for traits determining grain and stover yield in seven different field trials, distributed over 3 years and two seasons. The total genetic variation was partitioned into effects due to season (S), genotype (G), genotype x season interaction (G x S), and genotype x environment-within-season interaction [G x E(S)]. QTLs were determined for traits for their G, G x S, and G x E(S) effects, to assess the magnitude and the nature (cross over/non-crossover) of environmental interaction effects on individual QTLs. QTLs for some traits were associated with G effects only, while others were associated with the effects of both G and G x S and/or G, G x S and G x E(S) effects. The major G x S QTLs detected were for flowering time (on LG 4 and LG 6), and mapped to the same intervals as G x S QTLs for several other traits (including stover yield, harvest index, biomass yield and panicle number m(-2)). All three QTLs detected for grain yield were unaffected by G x S interaction however. All three QTLs for stover yield (mapping on LG 2, LG 4 and LG 6) and one of the three QTLs for grain yield (mapping on LG 4) were also free of QTL x E(S) interactions. The grain yield QTLs that were affected by QTL x E(S) interactions (mapping on LG 2 and LG 6), appeared to be linked to parallel QTL x E(S) interactions of the QTLs for panicle number m(-2) on (LG 2) and of QTLs for both panicle number m(-2) and harvest index (LG 6). In general, QTL x E(S) interactions were more frequently observed for component traits of grain and stover yield, than for grain or stover yield per se.
对由两个自交系H 77/833-2与PRLT 2/89-33杂交产生的104个珍珠粟F(3)株系组成的作图群体，以一个共同测验种进行测交，在7个不同的田间试验中评估了决定籽粒和秸秆产量的性状，这些试验分布在3年和两个季节。总的遗传变异被划分为季节（S）、基因型（G）、基因型×季节互作（G×S）以及基因型×季节内环境互作[G×E(S)]的效应。针对性状的G、G×S和G×E(S)效应确定了数量性状基因座（QTL），以评估环境互作对单个QTL的效应大小和性质（交叉/非交叉）。一些性状的QTL仅与G效应相关，而其他性状的QTL则与G和G×S和/或G、G×S和G×E(S)效应相关。检测到的主要G×S QTL与开花时间有关（位于LG 4和LG 6上），并且与其他几个性状（包括秸秆产量、收获指数、生物量产量和每平方米穗数）的G×S QTL定位在相同区间。然而，检测到的所有三个籽粒产量QTL均不受G×S互作的影响。所有三个秸秆产量QTL（位于LG 2、LG 4和LG 6上）以及三个籽粒产量QTL中的一个（位于LG 4上）也不存在QTL×E(S)互作。受QTL×E(S)互作影响的籽粒产量QTL（位于LG 2和LG 6上），似乎与每平方米穗数（位于LG 2上）以及每平方米穗数和收获指数（位于LG 6上）的QTL的平行QTL×E(S)互作有关。一般来说，籽粒和秸秆产量组成性状比籽粒或秸秆产量本身更频繁地观察到QTL×E(S)互作。
