Umeda Makoto, Kobayashi Hiroaki, Takeuchi Yasuo, Hayashi Joichiro, Morotome-Hayashi Yoko, Yano Kazuko, Aoki Akira, Ohkusa Toshifumi, Ishikawa Isao
Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
J Periodontol. 2003 Jan;74(1):129-34. doi: 10.1902/jop.2003.74.1.129.
Helicobacter pylori has been associated with the development of peptic ulcers and gastric cancer. Although it may be transmitted through the oral cavity, it is unknown whether the oral cavity acts as a permanent reservoir for this bacterium. The purpose of this study was to use nested polymerase chain reaction (PCR) to clarify whether the oral cavity acts as a reservoir for H. pylori.
The existence of H. pylori in the oral cavity was determined by nested PCR in 57 subjects and by culture method in 18 subjects. The presence of periodontopathic bacteria was also determined by 16S rRNA-based PCR method.
Although H. pylori was rarely detected in the oral cavity by culture technique, it was frequently detected (35.1%) by nested PCR in the oral cavity, especially among periodontitis patients who had the bacterium in the gastrointestinal tract (46.4%). Among the subjects who harbored H. pylori in the stomach or duodenum, 41.2% of patients with periodontal pockets > or = 4 mm and 9.1% of subjects without pockets showed H. pylori in dental plaque, although a statistically significant difference was not observed. One patient who had periodontal pockets retained H. pylori in the oral cavity even after eradication of the bacterium from the stomach and duodenum. Most (8/10) of the patients who had H. pylori in dental plaque harbored Bacteroides forsythus in their oral cavities.
Close attention should be given to periodontitis patients who harbor H. pylori in the oral cavity.
幽门螺杆菌与消化性溃疡和胃癌的发生有关。虽然它可能通过口腔传播，但口腔是否作为这种细菌的永久储存库尚不清楚。本研究的目的是使用巢式聚合酶链反应（PCR）来阐明口腔是否作为幽门螺杆菌的储存库。
通过巢式PCR检测57名受试者口腔中幽门螺杆菌的存在情况，并通过培养法检测18名受试者口腔中幽门螺杆菌情况。还通过基于16S rRNA的PCR方法检测牙周病原菌的存在情况。
虽然通过培养技术在口腔中很少检测到幽门螺杆菌，但通过巢式PCR在口腔中经常检测到（35.1%），尤其是在胃肠道中有该细菌的牙周炎患者中（46.4%）。在胃或十二指肠中携带幽门螺杆菌的受试者中，41.2%的牙周袋≥4 mm的患者和9.1%的无牙周袋的受试者在牙菌斑中检测到幽门螺杆菌，尽管未观察到统计学上的显著差异。一名有牙周袋的患者即使在胃和十二指肠中的细菌根除后，口腔中仍保留有幽门螺杆菌。牙菌斑中有幽门螺杆菌的患者中，大多数（8/10）口腔中还携带福赛坦氏拟杆菌。
应密切关注口腔中携带幽门螺杆菌的牙周炎患者。
