Gebara E C E, Faria C M, Pannuti C, Chehter L, Mayer M P A, Lima L A P A
Department of Stomatology, Division of Periodontology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
J Clin Periodontol. 2006 May;33(5):329-33. doi: 10.1111/j.1600-051X.2006.00915.x.
The present study aimed to evaluate if the oral cavity of chronic periodontitis patients can harbor Helicobacter pylori after systemic eradication therapy.
Samples of 30 patients (15 with gingivitis and 15 with chronic periodontitis) positive for H. pylori in the stomach were evaluated. Samples were collected 3 months after triple systemic antibiotic therapy from saliva, microbiota from the dorsum of the tongue, supra- and sub-gingival plaque as well as gastric biopsies. DNA of each sample was extracted by the boiling method and used as a template in polymerase chain reaction with the primers JW22/23.
Eighteen patients (60%) harboured H. pylori in their mouths. Five patients (16.6%) were positive in saliva, two (6.6%) on the dorsum of the tongue, nine (30%) in supra-gingival plaque, 14 (46.6%) in sub-gingival plaque and three (10%) in the stomach. There was no statistically significant difference between study groups.
Eradication of H. pylori after therapy was more effective for the stomach than for the mouth (p<0.001). Mouths of patients with gingivitis or with chronic periodontitis, who are positive for H. pylori in their stomachs, may be considered as reservoirs of these bacteria.
本研究旨在评估慢性牙周炎患者在全身根除治疗后口腔中是否能携带幽门螺杆菌。
对30例胃内幽门螺杆菌检测呈阳性的患者（15例牙龈炎患者和15例慢性牙周炎患者）的样本进行评估。在三联全身抗生素治疗3个月后，从唾液、舌背微生物群、龈上和龈下菌斑以及胃活检组织中采集样本。每个样本的DNA通过煮沸法提取，并用作与引物JW22/23进行聚合酶链反应的模板。
18例患者（60%）口腔中携带幽门螺杆菌。5例患者（16.6%）唾液检测呈阳性，2例（6.6%）舌背检测呈阳性，9例（30%）龈上菌斑检测呈阳性，14例（46.6%）龈下菌斑检测呈阳性，3例（10%）胃检测呈阳性。研究组之间无统计学显著差异。
治疗后幽门螺杆菌的根除在胃部比在口腔更有效（p<0.001）。胃部幽门螺杆菌检测呈阳性的牙龈炎或慢性牙周炎患者的口腔可被视为这些细菌的储存库。
