Vandrey J P, Goldenberg C J, Eliceiri G L
Biochim Biophys Acta. 1976 Apr 15;432(1):104-12. doi: 10.1016/0005-2787(76)90046-0.
When cells were incubated with L-[35S] methionine plus L-[Me-3H] methionine, it was found that at least four ribosomal proteins had 3H/35S ratios higher than the rest of the ribosomal proteins, suggesting that they were methylated. The rate of apparent methylation paralleled the rate of amino acid incorporation. Amino acid incorporation into ribosomal proteins revealed several rapidly labeled components. When 3H-labeled amino acid incorporation was chased for 10 min with an excess of non-radioactive amino acids, several proteins reached at least 60% of the specific activity they showed after 150 min of chase. The time lapse between the onset of 3H-labeled amino acid incorporation and arrival at its plateau appeared to differ among various ribosomal proteins of a subunit, suggesting a heterogeneity in the pools of ribosomal proteins.
当细胞与L-[³⁵S]甲硫氨酸加L-[Me-³H]甲硫氨酸一起孵育时，发现至少有四种核糖体蛋白的³H/³⁵S比值高于其余核糖体蛋白，这表明它们被甲基化了。表观甲基化速率与氨基酸掺入速率平行。氨基酸掺入核糖体蛋白显示出几个快速标记的组分。当用过量的非放射性氨基酸追踪³H标记的氨基酸掺入10分钟时，几种蛋白质达到了它们在追踪150分钟后所显示的比活性的至少60%。一个亚基的各种核糖体蛋白之间，³H标记的氨基酸掺入开始到达到平台期之间的时间间隔似乎有所不同，这表明核糖体蛋白池存在异质性。
