酵母核糖体蛋白的修饰。甲基化。
Modification of yeast ribosomal proteins. Methylation.
作者信息
Kruiswijk T, Kunst A, Planta R J, Mager W H
出版信息
Biochem J. 1978 Oct 1;175(1):221-5. doi: 10.1042/bj1750221.
Abstract
Two-dimensional polyacrylamide-gel electrophoretic analysis of yeast ribosomal proteins uniformly labelled in vivo with [methyl-3H]methionine and [1-14C]methionine revealed that four ribosomal proteins are methylated, i.e. proteins S31, S32, L15 and L41. Lysine and arginine appear to be the predominant acceptors of the methyl groups. The degree of methylation ranges from 0.09 to 0.20 methyl group per modified ribosomal protein species.
摘要
对在体内用[甲基-³H]甲硫氨酸和[1-¹⁴C]甲硫氨酸均匀标记的酵母核糖体蛋白进行二维聚丙烯酰胺凝胶电泳分析，结果显示有四种核糖体蛋白被甲基化，即蛋白S31、S32、L15和L41。赖氨酸和精氨酸似乎是甲基的主要受体。每个修饰的核糖体蛋白种类的甲基化程度在0.09至0.20个甲基之间。
相似文献
1
Modification of yeast ribosomal proteins. Methylation.
Biochem J. 1978 Oct 1;175(1):221-5. doi: 10.1042/bj1750221.
2
Modification of yeast ribosomal proteins. Phosphorylation.
Biochem J. 1978 Oct 1;175(1):213-9. doi: 10.1042/bj1750213.
3
Methylated proteins and amino acids in the ribosomes of Saccharomyces cerevisiae.
Eur J Biochem. 1984 Jun 15;141(3):585-90. doi: 10.1111/j.1432-1033.1984.tb08233.x.
4
Methylation of ribosomal proteins in bacteria: evidence of conserved modification of the eubacterial 50S subunit.
J Bacteriol. 1984 Apr;158(1):84-93. doi: 10.1128/jb.158.1.84-93.1984.
5
A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeast.
J Biol Chem. 2005 Oct 14;280(41):34590-8. doi: 10.1074/jbc.M507672200. Epub 2005 Aug 11.
6
Methylated amino acids in the proteins of the cytoplasmic ribosome of Tetrahymena thermophila.
Biochimie. 1989 Jul;71(7):805-11. doi: 10.1016/0300-9084(89)90043-6.
7
Methylation of ribosomal proteins in Bacillus subtilis.
J Bacteriol. 1980 Apr;142(1):355-8. doi: 10.1128/jb.142.1.355-358.1980.
8
The yeast 5S rRNA binding ribosomal protein YL3 is phosphorylated in vivo.
Biochim Biophys Acta. 1990 Oct 23;1087(2):142-6. doi: 10.1016/0167-4781(90)90198-b.
9
The ribosomal l1 protuberance in yeast is methylated on a lysine residue catalyzed by a seven-beta-strand methyltransferase.
J Biol Chem. 2011 May 27;286(21):18405-13. doi: 10.1074/jbc.M110.200410. Epub 2011 Apr 1.
10
The course of the assembly of ribosomal subunits in yeast.
Biochim Biophys Acta. 1978 Feb 16;517(2):378-89. doi: 10.1016/0005-2787(78)90204-6.
引用本文的文献
1
Ribosomal Protein uS5 and Friends: Protein-Protein Interactions Involved in Ribosome Assembly and Beyond.
Biomolecules. 2023 May 18;13(5):853. doi: 10.3390/biom13050853.
2
Human PDCD2L Is an Export Substrate of CRM1 That Associates with 40S Ribosomal Subunit Precursors.
Mol Cell Biol. 2016 Nov 28;36(24):3019-3032. doi: 10.1128/MCB.00303-16. Print 2016 Dec 15.
3
Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).
J Biol Chem. 2014 Oct 31;289(44):30499-30510. doi: 10.1074/jbc.M114.601658. Epub 2014 Sep 17.
4
A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
PLoS Genet. 2013;9(1):e1003210. doi: 10.1371/journal.pgen.1003210. Epub 2013 Jan 17.
5
Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificity.
J Bacteriol. 2012 Dec;194(24):6917-26. doi: 10.1128/JB.01535-12. Epub 2012 Oct 19.
6
A methyltransferase-independent function for Rmt3 in ribosomal subunit homeostasis.
J Biol Chem. 2009 May 29;284(22):15026-37. doi: 10.1074/jbc.M109.004812. Epub 2009 Apr 9.
7
Protein arginine methyltransferases: from unicellular eukaryotes to humans.
Eukaryot Cell. 2007 Jun;6(6):889-98. doi: 10.1128/EC.00099-07. Epub 2007 Apr 27.
8
Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR.
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5942-7. doi: 10.1073/pnas.082119899.
9
High resolution mini-two-dimensional gel electrophoresis of yeast ribosomal proteins. A standard nomenclature for yeast ribosomal proteins.
Mol Biol Rep. 1981 Nov 30;8(1):37-44. doi: 10.1007/BF00798383.
10
Methylation of ribosomal proteins in Bacillus subtilis.
J Bacteriol. 1980 Apr;142(1):355-8. doi: 10.1128/jb.142.1.355-358.1980.
本文引用的文献
1
Amino acid sequence of a 50 S ribosomal protein involved in both EFG and EFT dependent GTP-hydrolysis.
FEBS Lett. 1972 Dec 15;28(3):325-328. doi: 10.1016/0014-5793(72)80742-7.
2
Characteristics of the methylation in vivo of ribosomal RNA in yeast.
Biochim Biophys Acta. 1969 Dec 16;195(2):370-80. doi: 10.1016/0005-2787(69)90643-1.
3
The methylation of lysine residues in protein.
J Biol Chem. 1966 Apr 25;241(8):1857-62.
4
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12. doi: 10.1016/0003-2697(70)90376-3.
5
Analysis of the methylation sites in yeast ribosomal RNA.
Eur J Biochem. 1973 Nov 15;39(2):325-33. doi: 10.1111/j.1432-1033.1973.tb03130.x.
6
Methylation of ribosomal proteins in Escherichia coli.
J Bacteriol. 1974 Nov;120(2):651-6. doi: 10.1128/jb.120.2.651-656.1974.
7
Methylation of ribosomal proteins in vitro.
Nature. 1974 Oct 25;251(5477):731-3. doi: 10.1038/251731a0.
8
Properties of ribosomes and RNA synthesized by Escherichia coli grown in the presence of ethionine. 3. Methylated proteins in 50 S ribosomes of E. coli EA2.
J Mol Biol. 1974 Jun 15;86(1):139-59. doi: 10.1016/s0022-2836(74)80013-6.
9
50-S ribosomal proteins. Peptide studies on two acidic proteins, A 1 and A 2 , isolated from 50-S ribosomes of Escherichia coli.
Eur J Biochem. 1972 Jan 31;25(1):13-9. doi: 10.1111/j.1432-1033.1972.tb01661.x.
10
Analysis of the protein composition of yeast ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
Mol Biol Rep. 1974 Sep;1(7):409-15. doi: 10.1007/BF00385674.
Zotero 插件