Clarke D E, Romanyshyn W M
Br J Pharmacol. 1976 Mar;56(3):271-7. doi: 10.1111/j.1476-5381.1976.tb07638.x.
1 A new method for studying adrenal medullary release is described in pithed rats using field stimulation of an entire adrenal gland. 2 The increases in blood pressure in response to field stimulation of the gland consisted of an initial short, variable component, due to stimulation of adrenergic vasomotor neurones and a secondary, longer lasting component, due to medullary catecholamine release. 3 Removal of the initial component by drugs or cardiac/coeliac ganglionectomy did not affect the magnitude of the secondary pressor component. 4 The secondary pressor component was frequency-dependent, reproducible and stable with time. The extent of medullary catecholamine release could be assessed by comparing the blood pressure rise with those obtained after adrenaline injections. 5 The method appears to provide a reliable means by which adrenal medullary catecholamine release may be assessed without significant interference from vasomotor nerves.
本文描述了一种在去大脑大鼠中使用对整个肾上腺进行场刺激来研究肾上腺髓质释放的新方法。
对肾上腺进行场刺激时血压的升高包括一个初始的短暂、可变成分，这是由于肾上腺素能血管运动神经元受到刺激所致，以及一个继发的、持续时间更长的成分，这是由于髓质儿茶酚胺释放所致。
通过药物或心脏/腹腔神经节切除术去除初始成分并不影响继发升压成分的幅度。
继发升压成分与频率相关，可重复且随时间稳定。通过将血压升高与注射肾上腺素后获得的血压升高进行比较，可以评估髓质儿茶酚胺释放的程度。
该方法似乎提供了一种可靠的手段，通过它可以在不受血管运动神经显著干扰的情况下评估肾上腺髓质儿茶酚胺的释放。
