de Souza Trindade Giliane, da Fonseca Flávio Guimarães, Marques João Trindade, Nogueira Maurício Lacerda, Mendes Luiz Claudio Nogueira, Borges Alexandre Secorun, Peiró Juliana Regina, Pituco Edviges Maristela, Bonjardim Cláudio Antônio, Ferreira Paulo César Peregrino, Kroon Erna Geessien
Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Emerg Infect Dis. 2003 Feb;9(2):155-60. doi: 10.3201/eid0902.020244.
We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
我们描述了一种类痘病毒——阿拉萨图巴病毒，它与奶牛群中发生的类似牛痘的疫情以及一例相关的人类感染病例有关。诊断基于病毒生长特性、电子显微镜检查和分子生物学技术。通过聚合酶链反应扩增、克隆以及对痘苗病毒生长因子（VGF）、胸苷激酶（TK）和血凝素等保守正痘病毒基因进行DNA测序，对该病毒进行了分子特征分析。我们使用VGF同源基因和TK基因核苷酸序列构建系统发育树，以便与其他痘病毒进行比较。基因序列与痘苗病毒基因显示出99%的同源性，并在系统发育树中与分离出的病毒聚集在一起。阿拉萨图巴病毒与坎塔加洛病毒非常相似，在基因中显示出相同的特征性缺失。阿拉萨图巴病毒可能是一种新型类痘病毒，也可能代表坎塔加洛病毒的传播。
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