Murtoniemi T, Hirvonen M-R, Nevalainen A, Suutari M
National Public Health Institute, Division of Environmental Health, Kuopio, Finland.
Indoor Air. 2003 Mar;13(1):65-73. doi: 10.1034/j.1600-0668.2003.01126.x.
Microbial growth on water-damaged building materials is commonly associated with adverse health effects in the occupants. We examined the growth of Stachybotrys chartarum, Aspergillus versicolor, Penicillium spinulosum, and Streptomyces californicus, isolated from water-damaged buildings, on six different brands of plasterboards. The microbial growth was compared with the biological activity of the spores, that is the potential to induce cytotoxicity and proinflammatory mediators in RAW264.7 macrophages. These results showed that the microbial growth on plasterboard depended on both the microbial strain and the brand of plasterboard used. The biological activity of spores appeared to be regulated by different growth conditions on plasterboards so that good microbial growth was associated with a low bioactivity of the spores, whereas the spores collected from plasterboard supporting only weak growth usually were biologically active. Cytotoxicity of either S. chartarum or A. versicolor did not correlate with any particular growth conditions or induced inflammatory responses. Instead, there were positive correlations between cytotoxicity and levels of induced proinflammatory cytokines for P. spinulosum and S. californicus. These data suggest that both the microbial growth on plasterboard and the resulting bioactivity of spores vary and might be affected by changing the growth conditions provided by the plasterboards.
水浸损坏的建筑材料上的微生物生长通常与居住者的健康不良影响有关。我们研究了从水浸损坏建筑中分离出的黑曲霉、杂色曲霉、小刺青霉和加利福尼亚链霉菌在六种不同品牌石膏板上的生长情况。将微生物生长情况与孢子的生物活性进行了比较，即孢子在RAW264.7巨噬细胞中诱导细胞毒性和促炎介质的潜力。这些结果表明，石膏板上的微生物生长既取决于微生物菌株，也取决于所用石膏板的品牌。石膏板上不同的生长条件似乎调节了孢子的生物活性，因此良好的微生物生长与孢子的低生物活性相关，而从仅支持微弱生长的石膏板上收集的孢子通常具有生物活性。黑曲霉或杂色曲霉的细胞毒性与任何特定的生长条件或诱导的炎症反应均无关联。相反，小刺青霉和加利福尼亚链霉菌的细胞毒性与诱导的促炎细胞因子水平之间存在正相关。这些数据表明，石膏板上的微生物生长以及由此产生的孢子生物活性各不相同，并且可能会受到改变石膏板所提供生长条件的影响。
