Panyam Jayanth, Labhasetwar Vinod
Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Adv Drug Deliv Rev. 2003 Feb 24;55(3):329-47. doi: 10.1016/s0169-409x(02)00228-4.
Biodegradable nanoparticles formulated from poly (D,L-lactide-co-glycolide) (PLGA) have been extensively investigated for sustained and targeted/localized delivery of different agents including plasmid DNA, proteins and peptides and low molecular weight compounds. Research about the mechanism of intracellular uptake of nanoparticles, their trafficking and sorting into different intracellular compartments, and the mechanism of enhanced therapeutic efficacy of nanoparticle-encapsulated agent at cellular level is more recent and is the primary focus of the review. Recent studies in our laboratory demonstrated rapid escape of PLGA nanoparticles from the endo-lysosomal compartment into cytosol following their uptake. Based on the above mechanism, various potential applications of nanoparticles for delivery of therapeutic agents to the cells and tissue are discussed.
由聚（D，L-丙交酯-共-乙交酯）（PLGA）制备的可生物降解纳米颗粒已被广泛研究用于不同药物的持续和靶向/局部递送，这些药物包括质粒DNA、蛋白质、肽以及低分子量化合物。关于纳米颗粒细胞内摄取机制、其在不同细胞内区室中的运输和分选，以及细胞水平上纳米颗粒包封药物增强治疗效果的机制的研究是最近才开展的，也是本综述的主要重点。我们实验室最近的研究表明，PLGA纳米颗粒摄取后能迅速从内溶酶体区室逃逸到细胞质中。基于上述机制，讨论了纳米颗粒在将治疗药物递送至细胞和组织方面的各种潜在应用。
